胸腺素的免疫化学研究：胸腺素β4的放射免疫测定

Immunochemical studies on thymosin: radioimmunoassay of thymosin beta 4.

作者信息

Naylor P H, McClure J E, Spangelo B L, Low T L, Goldstein A L

出版信息

Immunopharmacology. 1984 Feb;7(1):9-16. doi: 10.1016/0162-3109(84)90003-1.

Abstract

Thymosin beta 4, a peptide with hormonal-like properties first isolated from the thymus gland, can be measured in serum using a newly described radioimmunoassay. The radioimmunoassay utilizes an antibody raised in rabbits against synthetic thymosin beta 4 conjugated by glutaraldehyde to keyhole limpet hemocyanin. A 125I-tyrosine-C13 analogue of the biologically active C-terminal fragment is used as the radioactive tracer. The radioimmunoassay is sensitive in the nanogram range and no cross-reactivity with common serum proteins is demonstrable. High performance liquid chromatography of serum samples indicates that two thymosin beta 4 cross-reactive species are present in human serum. Levels in serum range from 450 to 1100 ng/ml and decline with age.

摘要

胸腺素β4是一种最初从胸腺中分离出来的具有类激素特性的肽，可以使用一种新描述的放射免疫分析法在血清中进行检测。该放射免疫分析法利用在兔子体内产生的针对通过戊二醛与钥孔血蓝蛋白偶联的合成胸腺素β4的抗体。生物活性C末端片段的125I-酪氨酸-C13类似物用作放射性示踪剂。该放射免疫分析法在纳克范围内具有敏感性，并且与常见血清蛋白没有交叉反应性。血清样本的高效液相色谱分析表明，人血清中存在两种与胸腺素β4交叉反应的物质。血清中的水平范围为450至1100纳克/毫升，并随年龄增长而下降。