Goodall G J, Hempstead J L, Morgan J I
J Immunol. 1983 Aug;131(2):821-5.
Thymosin beta 4 antibodies have been raised in rabbits by conjugating the peptide to keyhole limpet hemocyanin, and a radioimmunoassay for thymosin beta 4 has been established utilizing tritiated thymosin beta 4 as binding ligand. RIA values for thymosin beta 4 in rat tissues reveal levels ranging between 8 and 448 micrograms/g wet weight. Highest levels are observed in spleen, although the peptide is also relatively abundant in thymus, lung, and nasal epithelium. Brain, kidney, liver and testis contain only 10 to 20% the concentration of thymosin beta 4 seen in spleen, whereas heart and muscle contain only 5 to 10% that of spleen. However, thymosin beta 4 probably has a ubiquitous distribution in the rat, being present in all tissues examined. The specificity of the antiserum used in the RIA has been defined. The thymosin beta 4 molecule seemingly bears two major epitopes that lie between amino acid residues 1-8 and 22-32. The antiserum does not cross-react with other unrelated peptides. The antiserum will precipitate biosynthetically labeled beta 4 from cultured rat peritoneal macrophage.
通过将胸腺素β4肽与钥孔血蓝蛋白偶联，在兔体内产生了胸腺素β4抗体，并利用氚标记的胸腺素β4作为结合配体建立了胸腺素β4的放射免疫测定法。大鼠组织中胸腺素β4的放射免疫测定值显示，其水平在8至448微克/克湿重之间。在脾脏中观察到最高水平，尽管该肽在胸腺、肺和鼻上皮中也相对丰富。脑、肾、肝和睾丸中胸腺素β4的浓度仅为脾脏中的10%至20%，而心脏和肌肉中仅为脾脏的5%至10%。然而，胸腺素β4可能在大鼠体内广泛分布，在所检查的所有组织中均有存在。已确定放射免疫测定中使用的抗血清的特异性。胸腺素β4分子似乎有两个主要表位，分别位于氨基酸残基1 - 8和22 - 32之间。该抗血清与其他无关肽无交叉反应。该抗血清将沉淀来自培养的大鼠腹腔巨噬细胞的生物合成标记的β4。
