Hanci D, Ulusoy S, Acar M, Burukoglu D, Kutlu H M, Bayar Muluk N, Cingi C
ENT Clinics, Okmeydani Training and Research Hospital, Istanbul, Turkey.
Eur Rev Med Pharmacol Sci. 2016 Aug;20(16):3469-75.
To investigate the potential preventive effect of resveratrol in rats exposed to acoustic trauma (AT).
In this experimental study, Wistar albino rats were divided into three groups: Group 1 (Control, n = 6), Group 2 (AT, n = 6), and Group 3 (resveratrol + AT). The rats in Group 2 were exposed to AT. The rats in Group 3 received resveratrol (300 mg/kg/day) via gavage for 7 days. On day 7, the rats were exposed to AT 10 min following resveratrol treatment. Histological sections of the cochleae were examined using light microscopy, transmission (TEM), and scanning electron microscopy (SEM).
The cochlear hair cells, stereocilia, and Deiters' cells of the control group appeared normal in all microscopic evaluations. In Group 2, light microscopy revealed predominantly inner hair cell loss, although the outer hair cells were affected. TEM and SEM examination showed severe loss of stereocilia and SEM revealed stereocilia arranged in an asymmetric array. The cochlear structure in Group 3 appeared well preserved under the light microscope, and although TEM and SEM revealed stereocilia loss, the hair cells and stereocilia appeared near normal compared with those of Group 2.
Resveratrol may have a protective effect against AT damage in the cochlea, most likely through its antioxidant activity. Our results may be useful for studies in humans exposed to AT and noise-induced hearing loss related to chronic exposure to occupational noise.
研究白藜芦醇对遭受声创伤（AT）大鼠的潜在预防作用。
在本实验研究中，将Wistar白化大鼠分为三组：第1组（对照组，n = 6）、第2组（AT组，n = 6）和第3组（白藜芦醇 + AT组）。第2组大鼠遭受声创伤。第3组大鼠通过灌胃给予白藜芦醇（300 mg/kg/天），持续7天。在第7天，大鼠在接受白藜芦醇治疗10分钟后遭受声创伤。使用光学显微镜、透射电子显微镜（TEM）和扫描电子显微镜（SEM）检查耳蜗的组织学切片。
在所有显微镜评估中，对照组的耳蜗毛细胞、静纤毛和代特细胞均正常。在第2组中，光学显微镜显示主要是内毛细胞损失，尽管外毛细胞也受到影响。TEM和SEM检查显示静纤毛严重损失，SEM显示静纤毛排列不对称。在光学显微镜下，第3组的耳蜗结构似乎保存完好，尽管TEM和SEM显示静纤毛损失，但与第2组相比，毛细胞和静纤毛接近正常。
白藜芦醇可能对耳蜗的声创伤损伤具有保护作用，最有可能是通过其抗氧化活性。我们的结果可能有助于对遭受声创伤以及与长期职业噪声暴露相关的噪声性听力损失的人类进行研究。
