Ada Servet, Hanci Deniz, Ulusoy Seçkin, Vejselova Djanan, Burukoglu Dilek, Muluk Nuray Bayar, Cingi Cemal
Luleburgaz State Hospital, ENT Department, Kırklareli, Turkey.
Okmeydanı Training and Research Hospital, ENT Clinics, Istanbul,Turkey.
Adv Clin Exp Med. 2017 Sep;26(6):893-897. doi: 10.17219/acem/64332.
Oxidative stress has been associated with pathological processes involved in acoustic trauma.
In this prospective experimental study, we investigated the potential preventive effect of N-acetyl cysteine (NAC) in rats exposed to acoustic trauma (AT). Light microscopic and scanning electron microscopic (SEM) evaluations were performed.
Healthy Wistar albino rats (n = 18) were divided into 3 groups: group 1 (control group, n = 6), group 2 (acoustic trauma group, n = 6), and group 3 (AT+NAC group, n = 6). The rats in group 2 were exposed to AT. The rats in group 3 received NAC at a dose of 100 mg/kg/day by gavage for 7 days, and then 10 min after the 7th-day dose, they were exposed to AT.
From light and scanning electron microscopy evaluations in the control group, the cochlear structure and epithelium were normal. In group 2 (AT group), extensive hair cell loss was observed in the cochlea by light microscopy evaluation. In the SEM evaluation, various epithelial damage and loss of stereocilia were also observed. In group 3 (AT+NAC group), decreased damage with preserved cochlear structures was seen by light microscopy. In the SEM evaluation, although stereocilia loss was also seen, nearly normal cell structures and vertical and symmetrical alignment of stereocilia structures were observed compared to the AT group.
NAC reduced cochlear damage due to acoustic trauma. Because NAC has antioxidant capacity, AT mat have caused an increase in free radicals and death of outer hair cells. NAC is an antioxidant agent and it prevented cochlear damage due to AT in rats.
氧化应激与声创伤所涉及的病理过程相关。
在这项前瞻性实验研究中，我们调查了N-乙酰半胱氨酸（NAC）对暴露于声创伤（AT）的大鼠的潜在预防作用。进行了光镜和扫描电子显微镜（SEM）评估。
将18只健康的Wistar白化大鼠分为3组：第1组（对照组，n = 6），第2组（声创伤组，n = 6），第3组（AT + NAC组，n = 6）。第2组的大鼠暴露于AT。第3组的大鼠通过灌胃给予剂量为100 mg/kg/天的NAC，持续7天，然后在第7天给药后10分钟，将它们暴露于AT。
通过对照组的光镜和扫描电子显微镜评估，耳蜗结构和上皮正常。在第2组（AT组）中，通过光镜评估观察到耳蜗中有广泛的毛细胞损失。在扫描电子显微镜评估中，还观察到各种上皮损伤和静纤毛损失。在第3组（AT + NAC组）中，通过光镜观察到损伤减轻，耳蜗结构得以保留。在扫描电子显微镜评估中，虽然也观察到静纤毛损失，但与AT组相比，观察到细胞结构几乎正常，静纤毛结构垂直且对称排列。
NAC减少了声创伤引起的耳蜗损伤。由于NAC具有抗氧化能力，AT可能导致自由基增加和外毛细胞死亡。NAC是一种抗氧化剂，它预防了大鼠因AT引起的耳蜗损伤。
