Axelrod D E, Gopalakrishnan T V, Willing M, Anderson W F
Somatic Cell Genet. 1978 Mar;4(2):157-68. doi: 10.1007/BF01538981.
It has previously been reported that Friend mouse erythroleukemia (MEL) cells synthesize hemoglobin when exposed to 2% dimethylsulfoxide, and that hybrids between MEL cells and fibroblasts (or other nonerythroid cells) do not synthesize hemoglobin. We have been successful in obtaining hybrids (3/15) between MEL cells and mouse L-cell fibroblasts that maintain hemoglobin inducibility by preserving nonadherent cells after fusion. The proportion of hemoglobin inducible hybrids can be increased (8/11) by using a stable 2S (pseudotetraploid) MEL parent in addition to preserving nonadherent cells after fusion. All hybrids which were nonadherent were hemoglobin inducible, and all hybrids which were adherent were not. Five nonadherent hybrid clones were analyzed from fusions between a stable 2S MEL parent and a human fibroblast (WI-38, VA-2). All these clones were inducible for hemoglobin. It is concluded that gene dosage is effective in increasing the proportion of hemoglobin inducible hybrids, but hybrid morphology is the phenotype characteristic that correlates most closely with expression of hemoglobin inducibility.
此前有报道称，Friend小鼠红白血病（MEL）细胞在暴露于2%二甲基亚砜时会合成血红蛋白，而MEL细胞与成纤维细胞（或其他非红细胞系细胞）之间的杂种细胞则不合成血红蛋白。我们成功获得了MEL细胞与小鼠L-细胞成纤维细胞之间的杂种细胞（15个中有3个），通过在融合后保留非贴壁细胞，这些杂种细胞维持了血红蛋白诱导能力。除了在融合后保留非贴壁细胞外，使用稳定的2S（假四倍体）MEL亲本可以提高血红蛋白诱导杂种细胞的比例（11个中有8个）。所有非贴壁杂种细胞都具有血红蛋白诱导能力，而所有贴壁杂种细胞则没有。从稳定的2S MEL亲本与人类成纤维细胞（WI-38，VA-2）的融合中分析了5个非贴壁杂种细胞克隆。所有这些克隆都可诱导合成血红蛋白。得出的结论是，基因剂量对提高血红蛋白诱导杂种细胞的比例有效，但杂种细胞形态是与血红蛋白诱导能力表达最密切相关的表型特征。
