通过与去核小鼠神经母细胞瘤或成纤维细胞的细胞质融合，使弗瑞德红白血病细胞中血红蛋白诱导性消失。
Extinction of hemoglobin inducibility in Friend erythroleukemia cells by fusion with cytoplasm of enucleated mouse neuroblastoma or fibroblast cells.
作者信息
Gopalakrishnan T V, Thompson E B, Anderson W F
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1642-6. doi: 10.1073/pnas.74.4.1642.
Abstract
Friend mouse erythroleukemia cells (T3c1-2 and its subline 5000) can be induced to synthesize hemoglobin after treatment with 1.5% (vol/vol) dimethylsulfoxide. When these cells are fused with nonerythroid cells (namely, mouse neuroblastoma or L cells) hemoglobin induction is extinguished. In order to determine if the nucleus of the nonerythroid cell is necessary for this extinction, fusions were performed between mouse erythroleukemia cells and enucleated neuroblastoma or L cells. Hemoglobin induction was reduced or eliminated in clones of these hybrids even after 6 months of continuous culture. These results suggest that the cytoplasm of nonerythroid cells contains factor(s) that extinguish hemoglobin inducibility in erythroleukemic cells and that this new phenotype can be inherited.
摘要
友系小鼠红白血病细胞（T3c1 - 2及其亚系5000）在用1.5%（体积/体积）二甲基亚砜处理后可被诱导合成血红蛋白。当这些细胞与非红系细胞（即小鼠神经母细胞瘤或L细胞）融合时，血红蛋白诱导作用消失。为了确定非红系细胞的细胞核对于这种消失是否必要，进行了小鼠红白血病细胞与去核神经母细胞瘤或L细胞之间的融合。即使在连续培养6个月后，这些杂种细胞的克隆中的血红蛋白诱导作用也降低或消除了。这些结果表明，非红系细胞的细胞质中含有使红白血病细胞中血红蛋白诱导能力消失的因子，并且这种新表型可以遗传。
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Extinction of hemoglobin inducibility in Friend erythroleukemia cells by fusion with cytoplasm of enucleated mouse neuroblastoma or fibroblast cells.通过与去核小鼠神经母细胞瘤或成纤维细胞的细胞质融合，使弗瑞德红白血病细胞中血红蛋白诱导性消失。
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