Thorén S A
Institute of Hygiene, University of Copenhagen, Denmark.
J Toxicol Environ Health. 1989;27(4):523-32. doi: 10.1080/15287398909531320.
By using an image-analyzer system, toxicological effects of different substances on nonconfluent cells in monolayers can be assessed. The total number of cells and the number of viable cells are semiautomatically counted. Fluorescein diacetate is used as the viability marker. The method was tested by using monolayers of alveolar macrophages from rabbits exposed to manganese dioxide particles. A dose-response curve was obtained from a series of experiments in which the response at a certain dose level can be obtained from cell-counting procedures of the duration of 1 h. The method can be used as a short-term test, testing potentially toxic substances including cytostatic drugs where the viability should be determined. Another example is further given of the use of this technique. The results obtained from it are compared with estimates of the percentage of viable cells assessed by extracellular lactate dehydrogenase from nonexposed and manganese dioxide particle-exposed monolayers of rabbit alveolar macrophages. The two methods both account well for the theoretical relation when the lactate dehydrogenase activity values are corrected for an inactivation of this enzyme with time.
通过使用图像分析系统，可以评估不同物质对单层非汇合细胞的毒理学效应。细胞总数和活细胞数通过半自动计数获得。荧光素二乙酸酯用作活力标记物。该方法通过使用暴露于二氧化锰颗粒的兔肺泡巨噬细胞单层进行了测试。通过一系列实验获得了剂量反应曲线，在这些实验中，可以从1小时时长的细胞计数程序中获得特定剂量水平下的反应。该方法可用作短期试验，用于测试潜在有毒物质，包括应测定活力的细胞抑制药物。还给出了该技术使用的另一个例子。将由此获得的结果与通过细胞外乳酸脱氢酶评估的未暴露和暴露于二氧化锰颗粒的兔肺泡巨噬细胞单层活细胞百分比估计值进行比较。当乳酸脱氢酶活性值随时间校正该酶的失活时，这两种方法都能很好地解释理论关系。
