Thorén S A, Lanke J
Institute of Hygiene, University of Copenhagen, Denmark.
J Toxicol Environ Health. 1989;27(4):509-22. doi: 10.1080/15287398909531319.
A simple, flexible, low-cost image analyzer system is presented for determination of the total number of cells and the number of viable cells in monolayers of nonconfluent cells. Viable cells were identified and counted using an immunofluorescence method using fluorescein diacetate. Alveolar macrophages from rabbits were used. The different possible counting procedures that could be performed are analyzed. The precision of the method of estimating the cell numbers is indicated by a coefficient of variation of 5%. The experimental procedure is given for a short-term toxicological in vitro test in which the semiautomatic counting procedures can be performed within 1.5 h.
本文介绍了一种简单、灵活、低成本的图像分析系统，用于测定非汇合细胞单层中的细胞总数和活细胞数。使用荧光素二乙酸酯的免疫荧光方法来识别和计数活细胞。采用了来自兔子的肺泡巨噬细胞。分析了可能执行的不同计数程序。估计细胞数量方法的精密度由5%的变异系数表示。给出了短期体外毒理学试验的实验程序，其中半自动计数程序可在1.5小时内完成。
