Hashemi Mohammad, Sandoughi Mahnaz, Fazeli Seyed Amirhossein, Bahari Gholamreza, Rezaei Maryam, Zakeri Zahra
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran.
Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran.
Adv Med. 2016;2016:4985745. doi: 10.1155/2016/4985745. Epub 2016 Aug 16.
Purpose/Background. Mounting evidence designates that HLA-G plays a role in the regulation of inflammatory processes and autoimmune diseases. There are controversial reports concerning the impact of HLA-G gene polymorphism on rheumatoid arthritis (RA). This study was aimed at examining the impact of 14 bp ins/del and +3142G>C polymorphism with susceptibility and early disease activity in RA patients in a sample of the Iranian population. Methods. This case-control study was done on 194 patients with RA and 158 healthy subjects. The HLA-G rs1063320 (+3142G>C) and rs66554220 (14 bp ins/del) variants were genotype by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFP) and PCR method, respectively. Results. The HLA-G +3142G>C polymorphism significantly decreased the risk of RA in codominant (OR = 0.61, 95% CI = 0.38-0.97, p = 0.038, GC versus GG; OR = 0.36, 95% CI = 0.14-0.92, p = 0.034, CC versus GG), dominant (OR = 0.56, 95% CI = 0.36-0.87, p = 0.011, GC + CC versus GG), and allele (OR = 0.58, 95% CI = 0.41-0.84, p = 0.004, C versus G) inheritance models tested. Our finding did not support an association between HLA-G 14 bp ins/del variant and risk/protection of RA. In addition, no significant association was found between the polymorphism and early disease activity. Conclusion. In summary, our results showed that HLA-G +3142G>C gene polymorphism significantly decreased the risk of RA in a sample of the Iranian population.
目的/背景。越来越多的证据表明，HLA-G在炎症过程和自身免疫性疾病的调节中发挥作用。关于HLA-G基因多态性对类风湿关节炎（RA）的影响，存在有争议的报道。本研究旨在探讨14 bp插入/缺失和+3142G>C多态性对伊朗人群样本中RA患者易感性和疾病早期活动的影响。方法。本病例对照研究对194例RA患者和158名健康受试者进行。HLA-G rs1063320（+3142G>C）和rs66554220（14 bp插入/缺失）变异分别通过聚合酶链反应-限制性片段长度多态性（PCR-RFP）和PCR方法进行基因分型。结果。HLA-G +3142G>C多态性在共显性（OR = 0.61，95% CI = 0.38 - 0.97，p = 0.038，GC对GG；OR = 0.36，95% CI = 0.14 - 0.92，p = 0.034，CC对GG）、显性（OR = 0.56，95% CI = 0.36 - 0.87，p = 0.011，GC + CC对GG）和等位基因（OR = 0.58，95% CI = 0.41 - 0.84，p = 0.004，C对G）遗传模型中显著降低了RA风险。我们的研究结果不支持HLA-G 14 bp插入/缺失变异与RA风险/保护之间存在关联。此外，未发现该多态性与疾病早期活动之间存在显著关联。结论。总之，我们的结果表明，HLA-G +3142G>C基因多态性在伊朗人群样本中显著降低了RA风险。
