评估HLA-G基因14碱基插入/缺失和+3142G>C多态性与复发性自然流产易感性的关系。
Evaluation of HLA-G 14-bp ins/del and +3142G>C polymorphisms with susceptibility to recurrent spontaneous abortion.
作者信息
Hashemi Mohammad, Mokhtari Mojgan, Khazaeian Safura, Bahari Gholamreza, Rezaei Maryam, Nakhaee Alireza, Taheri Mohsen
机构信息
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; Dept. of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Dept. of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
出版信息
Taiwan J Obstet Gynecol. 2017 Jun;56(3):276-280. doi: 10.1016/j.tjog.2017.04.002.
OBJECTIVE
HLA-G is critically important for successful implantation during pregnancy. Increasing evidence supposed that HLA-G plays a key role in tolerance of the semi-allogeneic graft in pregnancy by inhibiting the cytotoxic functions of T and NK cells. The present study aimed to evaluate the impact of HLA-G rs1063320 (+3142G>C) and 14-bp insertion (ins)/deletion (del) polymorphisms on recurrent spontaneous abortion (RSA).
MATERIALS AND METHODS
Genomic DNA from 93 RSA patients and 93 normal fertile women was isolated using the salting out method. Genotyping of HLA-G +3142G>C and 14-bp ins/del variants was done by polymerase chain reaction restriction fragment length polymorphism (PCR-RFP) and PCR method, respectively.
RESULTS
The HLA-G +3142G>C polymorphism increased the risk of RSA in codominant (OR = 2.39, 95%CI = 1.27-4.49, p = 0.010, GC vs GG; OR = 3.28, 95%CI = 1.16-9.72, p = 0.040, CC vs GG) and dominant (OR = 2.52, 95%CI = 1.37-4.64, p = 0.004, GC + CC vs GG) tested inheritance models. HLA-G rs1063320 C allele was associated with increased risk of RSA (OR = 1.84, 95%CI = 1.20-2.83, p = 0.007). The del/del genotype as well as del allele of 14-bp ins/del variant increased that risk of RSA (OR = 3.02, 95%CI = 1.23-7.41, p = 0.025 and OR = 1.65, 95%CI = 1.09-2.50, p = 0.022, respectively).
CONCLUSION
In summary, our results showed that HLA-G gene polymorphisms significantly increased the risk of RSA in a sample of the Iranian population.
目的
HLA - G对孕期成功着床至关重要。越来越多的证据表明，HLA - G通过抑制T细胞和NK细胞的细胞毒性功能，在孕期半同种异体移植物的耐受性中起关键作用。本研究旨在评估HLA - G rs1063320（+3142G>C）和14碱基插入（ins）/缺失（del）多态性对复发性自然流产（RSA）的影响。
材料与方法
采用盐析法从93例RSA患者和93例正常育龄妇女中分离基因组DNA。分别采用聚合酶链反应 - 限制性片段长度多态性（PCR - RFP）和PCR方法对HLA - G +3142G>C和14碱基ins/del变异进行基因分型。
结果
在共显性（OR = 2.39，95%CI = 1.27 - 4.49，p = 0.010，GC与GG相比；OR = 3.28，95%CI = 1.16 - 9.72，p = 0.040，CC与GG相比）和显性（OR = 2.52，95%CI = 1.37 - 4.64，p = 0.004，GC + CC与GG相比）遗传模型中，HLA - G +3142G>C多态性增加了RSA的风险。HLA - G rs1063320 C等位基因与RSA风险增加相关（OR = 1.84，95%CI = 1.20 - 2.83，p = 0.007）。14碱基ins/del变异的del/del基因型以及del等位基因增加了RSA风险（分别为OR = 3.02，95%CI = 1.23 - 7.41，p = 0.025和OR = 1.65，95%CI = 1.09 - 2.50，p = 0.022）。
结论
总之，我们的结果表明，在伊朗人群样本中，HLA - G基因多态性显著增加了RSA风险。
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