中国荷斯坦公牛精液性状的全基因组关联研究

Genome-wide association study for semen traits of the bulls in Chinese Holstein.

作者信息

Qin Chunhua, Yin Hongwei, Zhang Xu, Sun Dongxiao, Zhang Qin, Liu Jianfeng, Ding Xiangdong, Zhang Yi, Zhang Shengli

机构信息

College of Animal Science and Technology, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, National Engineering Laboratory of Animal Breeding, China Agricultural University, Beijing, 100193, China.

出版信息

Anim Genet. 2017 Feb;48(1):80-84. doi: 10.1111/age.12433. Epub 2016 Sep 9.

DOI:10.1111/age.12433

PMID:27610941

Abstract

A genome-wide association study (GWAS) was performed to identify markers and candidate genes for five semen traits in the Holstein bull population in China. The analyzed dataset consisted of records from 692 bulls from eight bull stations; each bull was genotyped using the Illumina BovineSNP50 BeadChip. Association tests between each trait and the 41 188 informative high-quality SNPs were achieved with gapit software. In total, 19 suggestive significant SNPs, partly located within the reported QTL regions or within or close to the reported candidate genes, associated with five semen traits were detected. By combining our GWAS results with the biological functions of these genes, eight novel promising candidate genes, including ETNK1, PDE3A, PDGFRB, CSF1R, WT1, DSCAML1, SOD1 and RUNX2, were identified that potentially relate to semen traits. Our findings may provide a basis for further research on the genetic mechanism of semen traits and marker-assisted selection of such traits in Holstein bulls.

摘要

开展了一项全基因组关联研究（GWAS），以鉴定中国荷斯坦公牛群体中5种精液性状的标记和候选基因。分析的数据集由来自8个公牛站的692头公牛的记录组成；每头公牛都使用Illumina BovineSNP50 BeadChip进行基因分型。使用gapit软件对每个性状与41188个信息丰富的高质量单核苷酸多态性（SNP）进行关联测试。总共检测到19个与5种精液性状相关的潜在显著SNP，部分位于已报道的数量性状位点（QTL）区域内，或位于已报道的候选基因内部或附近。通过将我们的GWAS结果与这些基因的生物学功能相结合，鉴定出8个新的有前景的候选基因，包括ETNK1、PDE3A、PDGFRB、CSF1R、WT1、DSCAML1、SOD1和RUNX2，它们可能与精液性状相关。我们的研究结果可能为进一步研究荷斯坦公牛精液性状的遗传机制以及此类性状的标记辅助选择提供基础。