Shahbazi S, Moztarzadeh F, Sadeghi G Mir Mohamad, Jafari Y
Department of Medical Engineering, Amirkabir University of Technology, Tehran, Iran.
Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:1201-9. doi: 10.1016/j.msec.2016.08.035. Epub 2016 Aug 14.
A novel poly propylene fumarate (PPF)-based glue which is reinforced by nanobioactive glass (NBG) particles and promoted by hydroxyethyl methacrylate (HEMA) as crosslinker agent, was developed and investigated for bone-to-bone bonding applications. In-vitro bioactivity, biodegradability, biocompatibility, and bone adhesion were tested and the results have verified that it can be used as bone glue. In an in-vitro condition, the prepared nanocomposite (PPF/HEMA/NBG) showed improved adhesion to wet bone surfaces. The combined tension and shear resistance between two wet bone surfaces was measured, and its maximum value was 9±59MPa. To investigate the bioactivity and biodegradability of the nanocomposite, it has been immersed in simulated body fluid (SBF). After 14days exposure to SBF, a hydroxyapatite (HA) layer formed on the surface of the composite confirms the bioactivity of this material. In the XRD pattern of the nanocomposite surface, the HA characteristic diffraction peak at θ=26 and 31.8 were observed. Also, by monitoring the weight change after 8weeks immersion in SBF, the mass loss was about 16.46wt%. It has been confirmed that this nanocomposite is a biodegradable material. Also, bioactivity and biodegradability of nanocomposite have been proved by SEM images. It has been showed that by using NBG particles and HEMA precursor, mechanical properties increased significantly. The ultimate tensile strength (UTS) of nanocomposite which contains 20% NBG and the ratio of 70/30wt% PPF/HEMA (PHB.732) was approximately 62MPa, while the UTS in the pure PPF/HEMA was about 32MPa. High cell viability in this nanocomposite (MTT assays, 85-95%) can be attributed to the NBG nature which contains calcium phosphate and is similar to physiological environment. Furthermore, it possesses biomineralization and biodegradation which significantly affected by impregnation of hydrophilic HEMA in the PPF-based polymeric matrix. The results indicated that the new synthesized biodegradable PPF/HEMA/NBG composite is suitable for biomedical applications especially as biodegradable bone glue in orthopedic surgeries.
一种新型的基于聚丙交酯富马酸酯（PPF）的胶水被研发并用于骨对骨粘结应用的研究，该胶水由纳米生物活性玻璃（NBG）颗粒增强，并由甲基丙烯酸羟乙酯（HEMA）作为交联剂促进。对其体外生物活性、生物降解性、生物相容性和骨粘附性进行了测试，结果证实它可作为骨胶使用。在体外条件下，制备的纳米复合材料（PPF/HEMA/NBG）对湿骨表面的粘附性有所改善。测量了两个湿骨表面之间的组合拉伸和剪切阻力，其最大值为9±59MPa。为了研究纳米复合材料的生物活性和生物降解性，将其浸入模拟体液（SBF）中。暴露于SBF 14天后，复合材料表面形成的羟基磷灰石（HA）层证实了该材料的生物活性。在纳米复合材料表面的XRD图谱中，观察到在θ=26和31.8处的HA特征衍射峰。此外，通过监测在SBF中浸泡8周后的重量变化，质量损失约为16.46wt%。已证实该纳米复合材料是一种可生物降解的材料。SEM图像也证明了纳米复合材料的生物活性和生物降解性。结果表明，通过使用NBG颗粒和HEMA前体，机械性能显著提高。含有20% NBG且PPF/HEMA比例为70/30wt%的纳米复合材料（PHB.732）的极限拉伸强度（UTS）约为62MPa，而纯PPF/HEMA中的UTS约为32MPa。该纳米复合材料中高细胞活力（MTT测定，85 - 95%）可归因于NBG的性质，其含有磷酸钙且类似于生理环境。此外，它具有生物矿化和生物降解性，这受到亲水性HEMA在基于PPF的聚合物基质中的浸渍的显著影响。结果表明，新合成的可生物降解的PPF/HEMA/NBG复合材料适用于生物医学应用，特别是作为骨科手术中的可生物降解骨胶。
