Zhang Yong-Guang, Lu Xin-Hua, Ding Yan-Bo, Zhou Xing-Kui, Wang Hong-Fei, Guo Jian-Wei, Liu Yong-Hong, Duan Yan-Qing, Li Wen-Jun
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
New Drug Research & Development Center, North China Pharmaceutical Group Corporation, National Microbial Medicine Engineering & Research Center, Shijiazhuang 050015, PR China.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5129-5133. doi: 10.1099/ijsem.0.001483. Epub 2016 Sep 8.
An alkalitolerant actinomycete strain, designated EGI 80674T, was isolated from a rhizosphere soil of Halocnermumstrobilaceum (Pall.) Bieb in Xinjiang, north-west China and subjected to a taxonomic characterization using a polyphasic approach. Strain EGI 80674T formed white aerial hyphae with long spore chains. Whole-cell hydrolysates of the isolate contained meso-diaminopimelic acid as the diagnostic diamino acid with no diagnostic sugars. The major fatty acids identified were iso-C16 : 0, anteiso-C17 : 0 and 10-methyl-C18 : 0TBSA. The predominant menaquinones detected were MK-10(H8) and MK-10(H6). The G+C content of the genomic DNA of strain EGI 80674T was 70.9 mol%. Strain EGI 80674T showed the highest 16S rRNA gene sequence similarity (97.24 %) to Nocardiopsis nikkonensis NBRC 102170T. The DNA-DNA relatedness value of strain EGI 80674T and N. nikkonensis NBRC 102170T was 18.4±1.3 %. Phenotypical, chemotaxonomic and phylogenetic characteristics and DNA-DNA hybridization data suggest that strain EGI 80674T represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis rhizosphaerae sp. nov. is proposed. The type strain is EGI 80674T (=CGMCC 4.7228T=KCTC 39673T).
从中国西北部新疆盐生肉苁蓉（Halocnermum strobilaceum (Pall.) Bieb）的根际土壤中分离出一株耐碱放线菌菌株，命名为EGI 80674T，并采用多相分类法对其进行分类鉴定。菌株EGI 80674T形成带有长孢子链的白色气生菌丝。该分离株的全细胞水解产物含有内消旋二氨基庚二酸作为诊断性二氨基酸，无诊断性糖。鉴定出的主要脂肪酸为异-C16 : 0、anteiso-C17 : 0和10-甲基-C18 : 0 TBSA。检测到的主要甲基萘醌为MK-10(H8)和MK-10(H6)。菌株EGI 80674T基因组DNA的G+C含量为70.9 mol%。菌株EGI 80674T与尼康诺卡氏菌（Nocardiopsis nikkonensis）NBRC 102170T的16S rRNA基因序列相似度最高（97.24 %）。菌株EGI 80674T与尼康诺卡氏菌NBRC 102170T的DNA-DNA相关性值为18.4±1.3 %。表型、化学分类和系统发育特征以及DNA-DNA杂交数据表明，菌株EGI 80674T代表诺卡氏菌属的一个新物种，为此提出新名称根际诺卡氏菌（Nocardiopsis rhizosphaerae）sp. nov.。模式菌株为EGI 80674T（=CGMCC 4.7228T=KCTC 39673T）。
