Han Jian, Gao Quan-Xiu, Zhang Yong-Guang, Li Li, Mohamad Osama A A, Rao Manik Prabhu Narsing, Xiao Min, Hozzein Wael N, Alkhalifah Dalal H M, Tao Yong, Li Wen-Jun
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, China.
Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2018 Mar 29;9:618. doi: 10.3389/fmicb.2018.00618. eCollection 2018.
The genus is an unique actinobacterial group that widely distributed in hypersaline environments. In this study, we investigated the growth conditions, transcriptome analysis, production and accumulation of ectoine by YIM 90087 under salt stress. The colony color of YIM 90087 changed from yellow to white under salt stress conditions. Accumulation of ectoine and hydroxyectoine in cells was an efficient way to regulate osmotic pressure. The ectoine synthesis was studied by transferring the related genes (, and ) to . Transcriptomic analysis showed that the pathways of ABC transporters (ko02010) and glycine, serine, and threonine metabolism (ko00260) played a vital role under salt stress environment. The from YIM 90087 was activated under the salt stress. Addition of exogenous ectoine and hydroxyectoine were helpful to protect YIM 90087 from salt stress.
该属是一类独特的放线菌，广泛分布于高盐环境中。在本研究中，我们调查了盐胁迫下YIM 90087的生长条件、转录组分析、四氢嘧啶的产生和积累。盐胁迫条件下，YIM 90087的菌落颜色从黄色变为白色。细胞内四氢嘧啶和羟基四氢嘧啶的积累是调节渗透压的有效方式。通过将相关基因（、和）转移到中研究了四氢嘧啶的合成。转录组分析表明，ABC转运蛋白（ko02010）和甘氨酸、丝氨酸及苏氨酸代谢（ko00260）途径在盐胁迫环境下起关键作用。YIM 90087的在盐胁迫下被激活。添加外源四氢嘧啶和羟基四氢嘧啶有助于保护YIM 90087免受盐胁迫。
