Odler B, Bikov A, Streizig J, Balogh C, Kiss E, Vincze K, Barta I, Horváth I, Müller V
1 Department of Pulmonology, Semmelweis University, Budapest, Hungary.
2 National Institute of Rheumatology and Physiotherapy, Budapest, Hungary.
Lupus. 2017 May;26(6):572-579. doi: 10.1177/0961203316668418. Epub 2016 Sep 10.
Biomarkers for pulmonary manifestations in systemic lupus erythematosus (SLE) are missing. Plasma samples of nine SLE patients with known pulmonary involvement (SLE) and nine SLE patients without pulmonary involvement (SLE) were tested by multiplex microarray analysis for various cyto- and chemokines. Significantly decreased lung function paramters for forced vital capacity (FVC), total lung capacity (TLC), diffusion capacity for carbon monoxide (DL) and diffusion of CO corrected on lung volume (KL) were observed in SLE as compared to SLE patients. CC chemokine ligand 21 (CCL21) and interferon gamma-induced protein 10 (IP-10) levels were significantly higher in SLE, than in patients without pulmonary manifestations. CCL21 correlated negatively with DL ( r = -0.73; p < 0.01) and KL ( r = -0.62; p < 0.01), while IP-10 with FVC and forced expiratory volume one second. Receiver Operating Characteristics (ROC) analysis confirmed high sensitivity and specificity for the separation of SLE patients with and without pulmonary involvement for the chemokines CCL21 (Area Under Curve (AUC): 0.85; sensitivity%: 88.90; specificity%: 75.00; p < 0.01) and IP-10 (AUC: 0.82; sensitivity%: 66.67, specificity%: 100; p < 0.01). Pleuropulmonary manifestations in SLE patients associated with lung functional and DL/KL changes and were associated with significant increase in CCL21 and IP-10. These chemokines might serve as potential biomarkers of lung involvement in SLE patients.
系统性红斑狼疮（SLE）肺部表现的生物标志物尚不清楚。通过多重微阵列分析检测了9例已知有肺部受累的SLE患者（SLE）和9例无肺部受累的SLE患者的血浆样本中的各种细胞因子和趋化因子。与无肺部受累的SLE患者相比，SLE患者的用力肺活量（FVC）、肺总量（TLC）、一氧化碳弥散量（DL）和基于肺容积校正的一氧化碳弥散率（KL）等肺功能参数显著降低。SLE患者的CC趋化因子配体21（CCL21）和干扰素γ诱导蛋白10（IP-10）水平显著高于无肺部表现的患者。CCL21与DL（r = -0.73；p < 0.01）和KL（r = -0.62；p < 0.01）呈负相关，而IP-10与FVC和一秒用力呼气量呈负相关。受试者工作特征（ROC）分析证实，趋化因子CCL21（曲线下面积（AUC）：0.85；敏感度%：88.90；特异度%：75.00；p < 0.01）和IP-10（AUC：0.82；敏感度%：66.67，特异度%：100；p < 0.01）在区分有和无肺部受累的SLE患者方面具有高敏感性和特异性。SLE患者的胸膜肺部表现与肺功能及DL/KL变化相关，并与CCL21和IP-10的显著升高相关。这些趋化因子可能是SLE患者肺部受累的潜在生物标志物。
