Adams K, Lafi A, Parry J M
Biomedical and Physiological Research Group, School of Biological Sciences, University College of Swansea, Great Britain.
Mutat Res. 1989 Aug;213(2):141-8. doi: 10.1016/0027-5107(89)90145-0.
The effects of 1,6-dinitropyrene (1,6-DNP) on the fidelity of cell division were studied in the transformed human fibroblast cell line MRC5VA. Over a dose range of 0.1-10 micrograms/ml of 1.6-DNP, we observed significant increases in the levels of abnormal division stages, associated with damage to the spindle apparatus of the cell. Qualitative changes in spindle morphology and a quantitative decrease in pole-to-pole spindle length were also observed with increasing doses of 1.6-DNP. Such changes in the size and morphology of the spindle corresponded with an accumulation of cells blocked at metaphase. The presence of catalase did not modify the response, suggesting that the effects on the spindle apparatus and cell division were not caused by the generation of radicals but by the direct action of 1.6-DNP.
在转化的人成纤维细胞系MRC5VA中研究了1,6 - 二硝基芘（1,6 - DNP）对细胞分裂保真度的影响。在0.1 - 10微克/毫升的1,6 - DNP剂量范围内，我们观察到异常分裂阶段水平显著增加，这与细胞纺锤体装置受损有关。随着1,6 - DNP剂量增加，还观察到纺锤体形态的定性变化以及极间纺锤体长度的定量减少。纺锤体大小和形态的这种变化与中期阻滞细胞的积累相对应。过氧化氢酶的存在并未改变这种反应，表明对纺锤体装置和细胞分裂的影响不是由自由基的产生引起的，而是由1,6 - DNP的直接作用引起的。
