1,6-二硝基芘可导致V79中国仓鼠细胞出现纺锤体紊乱和染色体损伤。

1,6-Dinitropyrene causes spindle disturbances and chromosomal damage in V79 Chinese hamster cells.

作者信息

Bauchinger M, Schmid E, Wiebel F J, Roscher E

机构信息

Institut für Strahlenbiologie, Umweltforschung Munich, Neuherberg, F.R.G.

出版信息

Mutat Res. 1988 Jul;208(3-4):213-8. doi: 10.1016/0165-7992(88)90063-2.

DOI:10.1016/0165-7992(88)90063-2

PMID:3398871

Abstract

We have investigated the cytogenetic effect of 1,6-dinitropyrene (1,6-DNP) in Chinese hamster V79 cells. The chemical caused a dose-dependent increase in the incidence of initial and full C-mitoses, polyploid mitoses, ana-telophases with lagging chromosomes, non-disjunction and multipolar configurations, in a range of 0.05-5 microM. These findings indicate that 1,6-DNP interferes with the functioning of the spindle apparatus in V79 cells. Early signs of spindle disturbances were seen at 1,6-DNP concentrations which only moderately reduced cell growth and division. Analysis of structural chromosomal aberrations revealed the appearance of chromatid-type aberrations with open breaks and exchanges accompanied by gaps. The results indicate that 1,6-DNP is both a spindle-disturbing and a clastogenic agent in V79 cells.

摘要

我们研究了1,6-二硝基芘（1,6-DNP）对中国仓鼠V79细胞的细胞遗传学效应。在0.05-5微摩尔的浓度范围内，该化学物质导致初期和完全C-有丝分裂、多倍体有丝分裂、伴有落后染色体的后期-末期、不分离和多极构型的发生率呈剂量依赖性增加。这些发现表明，1,6-DNP干扰了V79细胞中纺锤体装置的功能。在仅适度降低细胞生长和分裂的1,6-DNP浓度下，就观察到了纺锤体紊乱的早期迹象。对染色体结构畸变的分析显示，出现了具有开放断裂和交换并伴有间隙的染色单体型畸变。结果表明，1,6-DNP在V79细胞中既是一种纺锤体干扰剂，也是一种致断裂剂。