Du Gaoshang, Wang Lumei, Zhang Dongwei, Ni Xuan, Zhou Xiaotong, Xu Hanyi, Xu Lurong, Wu Shijian, Zhang Tong, Wang Wenhao
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Anal Biochem. 2016 Dec 1;514:2-7. doi: 10.1016/j.ab.2016.09.006. Epub 2016 Sep 9.
This paper proposes an aptasensor for progesterone (P4) detection in human serum and urine based on the aggregating behavior of gold nanoparticles (AuNPs) controlled by the interactions among P4-binding aptamer, target P4 and cationic surfactant hexadecyltrimethylammonium bromide (CTAB). The aptamer can form an aptamer-P4 complex with P4, leaving CTAB free to aggregate AuNPs in this aptasensor. Thus, the sensing solution will turn from red (520 nm) to blue (650 nm) in the presence of P4 because P4 aptamers are used up firstly owing to the formation of an aptamer-P4 complex, leaving CTAB free to aggregate AuNPs. However, in the absence of P4, CTAB combines with aptamers so that AuNPs still remain dispersed. Therefore, this assay makes it possible to detect P4 not only by absorbance measurement but also through naked eyes. By monitoring the variation of absorbance and color, a CTAB-induced colorimetric assay for P4 detection was established with a detection limit of 0.89 nM. Besides, the absorbance ratio A650/A520 has a linear correlation with the P4 concentration of 0.89-500 nM. Due to the excellent recoveries in serum and urine, this biosensor has great potential with respect to the visual and instrumental detection of P4 in biological fluids.
本文基于孕酮（P4）结合适配体、目标P4和阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）之间的相互作用所控制的金纳米颗粒（AuNPs）的聚集行为，提出了一种用于检测人血清和尿液中孕酮（P4）的适配体传感器。在该适配体传感器中，适配体可与P4形成适配体-P4复合物，使CTAB游离出来聚集AuNPs。因此，在存在P4的情况下，传感溶液会从红色（520nm）变为蓝色（650nm），因为由于形成了适配体-P4复合物，P4适配体首先被消耗殆尽，从而使CTAB游离出来聚集AuNPs。然而，在不存在P4的情况下，CTAB与适配体结合，使得AuNPs仍然保持分散状态。因此，该检测方法不仅可以通过吸光度测量，还可以通过肉眼检测P4。通过监测吸光度和颜色的变化，建立了一种CTAB诱导的比色法检测P4，检测限为0.89 nM。此外，吸光度比值A650/A520与0.89 - 500 nM的P4浓度具有线性相关性。由于在血清和尿液中具有出色的回收率，这种生物传感器在生物流体中P4的视觉和仪器检测方面具有巨大潜力。
