平车前多糖的优化及其抗氧化活性
Optimization and antioxidant activity of polysaccharides from Plantago depressa.
作者信息
Han Na, Wang Lin, Song Zehai, Lin Junyu, Ye Chun, Liu Zhihui, Yin Jun
机构信息
Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
出版信息
Int J Biol Macromol. 2016 Dec;93(Pt A):644-654. doi: 10.1016/j.ijbiomac.2016.09.028.
Polysaccharide from the herb of Plantago depressa (PDP) was obtained through ethanol precipitation preceded by a water extraction step. The optimum extraction yield of 5.68±0.46% was obtained with the treatment of raw material in water (w/v, 1:25.34) at 80.44°C during 1.97h, 3.28 times. Under these conditions, obtained yield value was in total agreement with value predicted by the model executed by Box-Behnken design (BBD). Following analysis by IR, HPLC-UV, MS and H NMR, the composition of PDP was found to be l-rhamnose, galactose, arabinose, glucose and d-galacturonic acid. The maximum tolerated dose of PDP was 10g/kg. The antioxidant activity of PDP was investigated using five tests and it was found that PDP was able to scavenge hydroxyl, DPPH and ABTS radicals, besides their β-carotene bleaching inhibitory activity. In particular, in the test of β-carotene bleaching inhibition, PDP displayed higher activity than Vitamin C.
车前草多糖（PDP）通过水提取步骤后乙醇沉淀获得。在80.44℃下，以水（w/v，1:25.34）处理原料1.97小时，提取率达到最佳值5.68±0.46%，是之前的3.28倍。在此条件下，获得的产率值与Box-Behnken设计（BBD）执行的模型预测值完全一致。经红外光谱（IR）、高效液相色谱-紫外检测（HPLC-UV）、质谱（MS）和核磁共振氢谱（H NMR）分析，发现PDP的组成成分为L-鼠李糖、半乳糖、阿拉伯糖、葡萄糖和D-半乳糖醛酸。PDP的最大耐受剂量为10g/kg。通过五项测试研究了PDP的抗氧化活性，发现PDP除了具有β-胡萝卜素漂白抑制活性外，还能够清除羟基、DPPH和ABTS自由基。特别是在β-胡萝卜素漂白抑制试验中，PDP表现出比维生素C更高的活性。
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