Box-Behnken 设计优化突尼斯变色藻（NCC 466）中粗多糖的提取：部分特性分析、体外抗氧化和抗菌活性。
Box-Behnken design for extraction optimization of crude polysaccharides from Tunisian Phormidium versicolor cyanobacteria (NCC 466): Partial characterization, in vitro antioxidant and antimicrobial activities.
机构信息
University of Sfax-Tunisia, Faculty of Sciences, Department of Life Sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and Planktonology, Street of Soukra Km 3.5, BP 1171, CP 3000 Sfax, Tunisia; University of Sfax-Tunisia, National School of Engineers, Engineering Laboratory of Environment and Ecotechnology, LR16ES19, Street of Soukra Km 3.5, BP 1173, CP 3038 Sfax, Tunisia.
University of Sfax-Tunisia, Faculty of Sciences, Department of Life Sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and Planktonology, Street of Soukra Km 3.5, BP 1171, CP 3000 Sfax, Tunisia.
出版信息
Int J Biol Macromol. 2017 Dec;105(Pt 2):1501-1510. doi: 10.1016/j.ijbiomac.2017.06.046. Epub 2017 Jun 9.
In this study, response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to optimize the aqueous extraction of crude polysaccharides from Tunisian cyanobacteria Phormidium versicolor (NCC 466). The optimal extraction conditions with an extraction yield of 21.56±0.92% were as follows: extraction temperature at 81.05°C, extraction time of 3.99h, and water to raw material ratio of 21.52mLg. Crude Phormidium versicolor polysaccharides (CPv-PS) are found to be a hetero-sulfated-anionic polysaccharides that contained carbohydrate (79.37±1.58%), protein (0.45±0.11%), uronic acids (4.37±0.19%) and sulfate (6.83±0.28%). The carbohydrate fraction was composed of arabinose, xylose, ribose, rhamnose, N-acetyl glucosamine, galactose, glucose, mannose, glucuronic acid and saccharose with corresponding mole percentages of 2.41, 14.58, 2.18, 6.23, 7.04, 28.21, 26.04, 3.02, 0.86 and 5.07, respectively. Evaluation of the antioxidant activity in vitro suggested that CPv-PS strongly scavenged radicals, prevented bleaching of β-carotene and reduced activity. Furthermore, the CPv-PS exhibited effective antimicrobial properties.
在这项研究中，采用基于 Box-Behnken 设计（BBD）的响应面法（RSM）来优化从突尼斯蓝藻藻 Phormidium versicolor（NCC 466）中提取粗多糖的水提条件。具有 21.56±0.92%提取率的最佳提取条件如下：提取温度为 81.05°C，提取时间为 3.99h，水与原料比为 21.52mL/g。发现粗 Phormidium versicolor 多糖（CPv-PS）是一种含有杂硫酸阴离子多糖，其含有碳水化合物（79.37±1.58%）、蛋白质（0.45±0.11%）、糖醛酸（4.37±0.19%）和硫酸盐（6.83±0.28%）。碳水化合物部分由阿拉伯糖、木糖、核糖、鼠李糖、N-乙酰葡萄糖胺、半乳糖、葡萄糖、甘露糖、葡萄糖醛酸和蔗糖组成，对应的摩尔百分比分别为 2.41%、14.58%、2.18%、6.23%、7.04%、28.21%、26.04%、3.02%、0.86%和 5.07%。体外抗氧化活性评价表明，CPv-PS 强烈清除自由基，防止β-胡萝卜素漂白，并具有还原活性。此外，CPv-PS 表现出有效的抗菌性能。
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