自动化显微镜定量高通量连续培养中的群体动力学。

Quantitative high-throughput population dynamics in continuous-culture by automated microscopy.

机构信息

Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Department of Physics, University of Illinois at Urbana-Champign, Urbana, IL 61801, USA.

出版信息

Sci Rep. 2016 Sep 12;6:33173. doi: 10.1038/srep33173.

DOI:10.1038/srep33173

PMID:27616752

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5018735/

Abstract

We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.

摘要

我们提出了一种高通量方法来测量连续培养中维持的微生物群落的丰度动态。我们的方法使用定制的 epi-fluorescence 显微镜，在精确控制培养条件的同时，自动对从连续培养群体中提取的单细胞进行成像。对于大肠杆菌的克隆群体，我们的仪器揭示了与历史相关的弹性和与生长速率相关的聚集。