School of Science and Technology, National University of San Martin, Buenos Aires, Argentina.
Department of Histology and Embryology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
J Biomed Mater Res B Appl Biomater. 2017 Nov;105(8):2408-2415. doi: 10.1002/jbm.b.33777. Epub 2016 Sep 12.
Although Ultrananocrystalline diamond (UNCD) has been proposed as a coating material for titanium biomedical implants, the biological effects and toxicity of UNCD particles that could eventually detach have not been studied to date. The biokinetics and biological effects of UNCD compared to titanium dioxide (TiO ) nanoparticles was evaluated in vivo using Wistar rats (n = 30) i.p. injected with TiO , UNCD or saline solution. After 6 months, blood, lung, liver, and kidney samples were histologically analyzed. Oxidative damage by membrane lipidperoxidation (thiobarbituric acid reactive substances-TBARS), generation of reactive oxygen species (superoxide anion- O2-), and antioxidant enzymes (superoxide dismutase-SOD, catalase-CAT) was evaluated in lung and liver. Histologic observation showed agglomerates of TiO or UNCD in the parenchyma of the studied organs, though there were fewer UNCD than TiO deposits. In addition, TiO caused areas compatibles with foci of necrosis in the liver and renal hyaline cylinders. Regarding UNCD, no membrane damage (TBARS) or mobilization of enzymatic antioxidants was observed either in lung or liver samples. No variations in O2- generation were observed in lung (Co: 35.1 ± 4.02 vs. UNCD: 48 ± 9.1, p > 0.05). Conversely, TiO exposure caused production of O2- in alveolar macrophages and consumption of catalase (p < 0.05). The studied parameters suggest that UNCD caused neither biochemical nor histological alterations, and therefore may prove useful as a surface coating for biomedical implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2408-2415, 2017.
虽然超纳米金刚石（UNCD）已被提议作为钛生物医学植入物的涂层材料，但迄今为止尚未研究最终可能脱落的 UNCD 颗粒的生物效应和毒性。本研究通过腹腔注射二氧化钛（TiO ）纳米颗粒、UNCD 或生理盐水的方式，在体内评估了 UNCD 与 TiO 纳米颗粒的生物动力学和生物效应，Wistar 大鼠（n = 30）为实验对象。6 个月后，对血液、肺、肝和肾组织样本进行组织学分析。评估了肺和肝中的膜脂质过氧化（硫代巴比妥酸反应物质-TBARS）、活性氧（超氧阴离子-O 2-）的产生以及抗氧化酶（超氧化物歧化酶-SOD、过氧化氢酶-CAT）的氧化损伤。研究结果表明，在所研究的器官的实质中，TiO 或 UNCD 聚集，但 UNCD 沉积物比 TiO 少。此外，TiO 导致肝和肾透明圆柱体中与坏死灶相容的区域。关于 UNCD，无论是在肺还是肝组织样本中，均未观察到膜损伤（TBARS）或抗氧化酶的移动。在肺中未观察到 O 2-生成的变化（Co：35.1 ± 4.02 与 UNCD：48 ± 9.1，p > 0.05）。相反，TiO 暴露导致肺泡巨噬细胞中 O 2-的产生和过氧化氢酶的消耗（p < 0.05）。研究参数表明，UNCD 既未引起生化改变，也未引起组织学改变，因此可能对生物医学植入物的表面涂层有用。 © 2016 Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料，105B：2408-2415，2017。
