Kikuchi K, Saito K, Suzuki M, Watanabe T
Shigaku. 1989 Feb;76(6):1096-165.
Since they are found to be increased in lesions of acute necrotic ulcerative gingivitis or marginal periodontitis, agents for these diseases. In the present study, 38 pure cultured strains were obtained as a result of isolation and culture of samples collected from lesions of marginal periodontitis (periodontal pokets), and the biological and biochemical characteristics of these strains were investigated. 1) Light microscopy (including dark-field microscopy) and transmission electron microscopy (negative staining) were used for observation of the morphology and cellular structure of the strains. The cells had a spiral shape, and showed active movement. Based on the above findings the cultured strains were all confirmed to be spirochetes of small to medium size, being 0.08-0.24 micron in width. 2) Growth and motility of the strains were investigated on various types of culture medium. Intense growth and movement were noted in strains cultured in bovine liver exudate medium containing horse serum (pH 7.2) at 37 degrees C under anaerobic conditions produced by the evacuation-replacement method (95% N2, 5% CO2) for 3-7 days after inoculation. 3) Thirty-five strains were positive for indole production and decomposition of urea, mucin, hippuric acid and esculin. Production of hydrogen sulfied was observed in 31 strains. In decomposition tests for 17 carbohydrates, 17 strains were positive for galactose and 14 strains were positive for glucose, while 11 strains were positive for dextrin and 10 strains for fructose upon decomposition of soluble starch. Other carbohydrates were also decomposed by a few strains. 4) In an investigation of the production of alcohol and lower fatty acids, among the metabolic products detected by gas chromatography, a large amount of acetic acid and small amounts of ethanol, lactic acid, propionic acid, pyruvic acid were observed. 5) The results of enzyme activity tests using an API ZYM system indicated relatively high activities of esterase, esterase-lipase, alpha-glucosidase, alkaline phosphatase, trypsin and acid phosphatase.
由于发现它们在急性坏死性溃疡性牙龈炎或边缘性牙周炎的病变中增多，故用于这些疾病的治疗。在本研究中，通过对从边缘性牙周炎（牙周袋）病变中采集的样本进行分离和培养，获得了38株纯培养菌株，并对这些菌株的生物学和生化特性进行了研究。1）使用光学显微镜（包括暗视野显微镜）和透射电子显微镜（负染色）观察菌株的形态和细胞结构。细胞呈螺旋状，并表现出活跃的运动。基于上述发现，培养的菌株均被确认为中小尺寸的螺旋体，宽度为0.08 - 0.24微米。2）在各种类型的培养基上研究菌株的生长和运动性。接种后，通过抽空 - 置换法（95% N2，5% CO2）在37℃厌氧条件下，于含马血清（pH 7.2）的牛肝渗出液培养基中培养3 - 7天，观察到菌株生长旺盛且运动活跃。3）35株菌株吲哚产生试验及尿素、粘蛋白、马尿酸和七叶苷分解试验呈阳性。31株菌株观察到硫化氢产生。在17种碳水化合物的分解试验中，17株菌株半乳糖分解试验呈阳性，14株菌株葡萄糖分解试验呈阳性，而11株菌株在可溶性淀粉分解时糊精分解试验呈阳性，10株菌株果糖分解试验呈阳性。其他碳水化合物也有少数菌株能分解。4）在对醇类和低级脂肪酸产生的研究中，通过气相色谱检测到的代谢产物中，观察到大量乙酸以及少量乙醇、乳酸、丙酸、丙酮酸。5）使用API ZYM系统进行酶活性测试的结果表明，酯酶、酯酶 - 脂肪酶、α - 葡萄糖苷酶、碱性磷酸酶、胰蛋白酶和酸性磷酸酶的活性相对较高。
