Fiehn N E, Westergaard J
Scand J Dent Res. 1986 Jun;94(3):208-18. doi: 10.1111/j.1600-0722.1986.tb01755.x.
The present investigation was carried out in order to obtain better information about the growth requirements for small-sized oral spirochetes containing two endoflagella from each cell-end. Eight strains of such spirochetes were isolated from subgingival plaque in patients suffering from advanced marginal periodontitis. The strains were maintained under anaerobic conditions in a fluid basal BHI medium with 15% inactivated rabbit serum, 0.07% Noble Agar and 5 micrograms/ml cocarboxylase. Firstly, the effect of trace amounts of oxygen in the atmosphere and pH in the medium on growth of the spirochete strains were examined. Secondly, the effect of different sera incorporated in the medium was examined, and thirdly, the effect of important growth factors in serum was studied by adding different serum components to the fluid basal medium instead of rabbit serum. Growth was always determined after 4 days' incubation at 35 degrees C, either by counting numbers of spirochete cells in a Petroff-Hauser counting chamber or by measuring the turbidity of the culture spectrophotometrically at 600 nm. There was no difference in growth by using an atmosphere containing 1% oxygen or an anaerobic atmosphere. It was found that serum (rabbit or human) was an essential growth component, and no single growth factor could replace rabbit serum. Only a long chain fatty acid mixture and an amino acid solution could, to a minor extent, stimulate growth compared to the basal medium without rabbit serum. Sodium bicarbonate inhibited growth of all strains. Finally, none of the strains fermented a series of low molecular weight carbohydrates, but all strains produced H2S and indole.
本次研究旨在获取更多关于小型口腔螺旋体生长需求的信息，这种螺旋体每个细胞末端含有两条内鞭毛。从患有晚期边缘性牙周炎患者的龈下菌斑中分离出八株此类螺旋体。这些菌株在厌氧条件下保存在含有15%灭活兔血清、0.07%诺布尔琼脂和5微克/毫升硫胺素焦磷酸的液体基础脑心浸液培养基中。首先，检测了大气中微量氧气和培养基pH值对螺旋体菌株生长的影响。其次，检测了培养基中加入不同血清的效果，第三，通过向液体基础培养基中添加不同血清成分而非兔血清来研究血清中重要生长因子的作用。在35℃孵育4天后，总是通过在血细胞计数板中计数螺旋体细胞数量或通过在600nm处用分光光度计测量培养物的浊度来测定生长情况。使用含1%氧气的气氛或厌氧气氛培养，生长情况没有差异。发现血清（兔血清或人血清）是必需的生长成分，没有单一生长因子能替代兔血清。与不含兔血清的基础培养基相比，只有长链脂肪酸混合物和氨基酸溶液能在较小程度上刺激生长。碳酸氢钠抑制所有菌株的生长。最后，所有菌株均不发酵一系列低分子量碳水化合物，但所有菌株均产生硫化氢和吲哚。
