基于聚集诱导发光的肝素选择性、可切换荧光探针
Selective, switchable fluorescent probe for heparin based on aggregation-induced emission.
作者信息
Wang Yi Jia, Lin Lei, Zhang Xing, Schultz Victor, Zhang Fuming, Sun Jing Zhi, Linhardt Robert J
机构信息
MoE Key Laboratory of Macromolecule Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China; Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States.
Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States.
出版信息
Anal Biochem. 2016 Dec 1;514:48-54. doi: 10.1016/j.ab.2016.09.007. Epub 2016 Sep 13.
A positively charged tetraphenylethene (TPE) derivative, TPE-4MN, was synthesized as a probe for heparin based on aggregation induced emission. On the addition of 5.0 μg/mL of heparin, TPE-4MN showed an enhanced emission of about 10-fold. The change in fluorescence at 475 nm was linear over a range of heparin concentrations of 0-1.0 μg/mL with an R = 0.99988 and the limit of detection (LOD) was calculated to be 0.75 μg/mL. The mechanism of the detection was proven to be through an ion pairing interaction. TPE-4MN showed good selectivity for heparin over other types of polysaccharides and could easily distinguish heparin from heparan sulfate, a glycosaminoglycan having a similar structure to that of heparin.
合成了一种带正电荷的四苯乙烯（TPE）衍生物TPE-4MN，作为基于聚集诱导发光的肝素探针。加入5.0μg/mL肝素后，TPE-4MN的发射增强了约10倍。在0-1.0μg/mL的肝素浓度范围内，475nm处荧光变化呈线性，R = 0.99988，计算出检测限（LOD）为0.75μg/mL。检测机制被证明是通过离子对相互作用。TPE-4MN对肝素的选择性优于其他类型的多糖，并且能够轻松区分肝素与硫酸乙酰肝素，后者是一种结构与肝素相似的糖胺聚糖。
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