[Study on the formation of experimental infected rabbit].

The purpose of the present study is to produce a model of infections of oral cavity by inducing abscess in the mandibla of New Zealand White rabbits as close to the oral cavity infection site as possible in order to determine whether the administered antimicrobial agents will transfer to the localized inflammatory foci. The pus to be collected should be 0.5 to 1.0g or more to permit bioassay and it should be collectable under nearly uniform conditions at all times. New Zealand White rabbit was the experimental animal used, in which anesthesia was induced intraperitoneally to expose the mandibula. A hole was made in the mandibula, into which a small cotton pellet inoculated with Streptococcus milleri and Bacteroides fragilis, whose cell concentrations had been adjusted to 10(8)CFU/ml, was implanted and the opening was carefully closed with sutures tightly. These two species of bacteria had been selected because mixtures of gram-positive cocci and anaerobic bacteria have been isolated in most cases from the occluded abscess of oral cavity infections. As a result of the above procedure, it was found that one week after production of the infected model animal, the abscess contained adequate volume of, pus for collection and demonstrated viability of the two transplanted species of bacteria. This animal model was considered an ideal model for use in the studies on treatment of infections in the oral cavity by administering antimicrobial agents to determine the extent of drug transfer into the pus and bones where purulent foci are present.