Lacroix J M, Mayrand D
Oral Microbiol Immunol. 1989 Jun;4(2):82-8. doi: 10.1111/j.1399-302x.1989.tb00104.x.
A test tube technique was developed to screen bacterial mixtures to detect interbacterial interactions that play a role in determining sensitivity to antimicrobial agents. We found 3 mixtures where these bacterial interactions change the sensitivity to antimicrobials or change the proportions of each bacterial species in the mixture. The mixtures were: Fusobacterium nucleatum 102.3 and Bacteroides endodontalis ATCC 35406; F. nucleatum 102.3 and B. endodontalis BN11 a-f; and Capnocytophaga ochracea 1956c and Eubacterium saburreum 162.4. The antimicrobials used were metronidazole for the first 2 mixtures and tetracycline for the last. F. nucleatum seems to protect B. endodontalis from the action of metronidazole. Conversely, the growth inhibition of C. ochracea by E. saburreum was lifted when tetracycline was present. We also found that the growth of C. ochracea can then permit the subsequent growth of E. saburreum. The test tube method permits the evaluation both of interbacterial interactions and the detection of any protective mechanism against antimicrobial agents in a bacterial mixture. We found that F. nucleatum 102.3 can decrease the metronidazole level in the culture medium, and by the use of 14C-metronidazole we demonstrated that acetamide is produced from metronidazole.
我们开发了一种试管技术，用于筛选细菌混合物，以检测在决定对抗菌剂敏感性方面起作用的细菌间相互作用。我们发现了3种混合物，其中这些细菌间相互作用改变了对抗菌剂的敏感性或改变了混合物中每种细菌的比例。这些混合物分别是：具核梭杆菌102.3和牙髓拟杆菌ATCC 35406；具核梭杆菌102.3和牙髓拟杆菌BN11 a - f；以及赭黄嗜二氧化碳噬纤维菌1956c和萨氏真杆菌162.4。前两种混合物使用甲硝唑作为抗菌剂，最后一种使用四环素。具核梭杆菌似乎能保护牙髓拟杆菌免受甲硝唑的作用。相反，当存在四环素时，萨氏真杆菌对赭黄嗜二氧化碳噬纤维菌的生长抑制作用解除。我们还发现，赭黄嗜二氧化碳噬纤维菌的生长随后能使萨氏真杆菌生长。这种试管方法既可以评估细菌间相互作用，也可以检测细菌混合物中任何针对抗菌剂的保护机制。我们发现具核梭杆菌102.3可以降低培养基中的甲硝唑水平，并且通过使用14C - 甲硝唑，我们证明了甲硝唑能产生乙酰胺。
