Singh Dhirendra Kumar, Gulati Kavita, Ray Arunabha
Department of Pharmacology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110 007, India.
Department of Pharmacology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110 007, India.
Int Immunopharmacol. 2016 Nov;40:229-234. doi: 10.1016/j.intimp.2016.08.009. Epub 2016 Sep 9.
The present study evaluated the immunomodulatory effects of chelidonic acid, a secondary plant metabolite, with therapeutic potential in allergic disorders, in experimental animals. In mast cell degranulation studies, ovalbumin immunized and challenged rats, chelidonic acid (1, 3 and 10mg/kg, i.p.) dose relatedly prevented ovalbumin challenge induced mast cell degranulation by differing degrees when compared with vehicle treated group, and these effects were comparable with prednisolone (10mg/kg). A reduction in post-challenge mortality was also observed in all treated groups. Further, there were reductions in the blood eosinophil counts and serum IgE levels after chelidonic acid treatment. Chelidonic acid also inhibited histamine release from rat peritoneal mast cells (RPMC) in vitro, in a dose related manner. In tests for adaptive immunity, in rats immunized with sheep RBC, chelidonic acid differentially suppressed the (a) plaque forming cell (PFC) count in rat splenic cells, (b) anti-SRBC antibody titre and serum IgG levels and (c) increases in foot pad thickness in the DTH assay - all of which were comparable with prednisolone. These experimental results are discussed in light of the possible therapeutic potential of chelidonic acid in allergic disorders.
本研究评估了白屈菜酸（一种具有治疗过敏性疾病潜力的植物次生代谢产物）对实验动物的免疫调节作用。在肥大细胞脱颗粒研究中，用卵清蛋白免疫并激发的大鼠，与赋形剂处理组相比，白屈菜酸（1、3和10mg/kg，腹腔注射）能不同程度地剂量依赖性地预防卵清蛋白激发诱导的肥大细胞脱颗粒，且这些作用与泼尼松龙（10mg/kg）相当。在所有处理组中还观察到激发后死亡率降低。此外，白屈菜酸处理后，血液嗜酸性粒细胞计数和血清IgE水平降低。白屈菜酸还能在体外以剂量依赖性方式抑制大鼠腹膜肥大细胞（RPMC）释放组胺。在适应性免疫测试中，在用绵羊红细胞免疫的大鼠中，白屈菜酸能不同程度地抑制：（a）大鼠脾细胞中的空斑形成细胞（PFC）计数；（b）抗SRBC抗体滴度和血清IgG水平；以及（c）迟发型超敏反应（DTH）试验中足垫厚度的增加——所有这些都与泼尼松龙相当。根据白屈菜酸在过敏性疾病中的可能治疗潜力对这些实验结果进行了讨论。
