预测真核生物跨膜蛋白的方向。
Predicting the orientation of eukaryotic membrane-spanning proteins.
作者信息
Hartmann E, Rapoport T A, Lodish H F
机构信息
Central Institute for Molecular Biology, Academy of Sciences of the German Democratic Republic, Berlin-Buch.
出版信息
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5786-90. doi: 10.1073/pnas.86.15.5786.
Abstract
We have developed a rule to predict the orientation of the first internal signal-anchor sequence in eukaryotic transmembrane proteins synthesized on the rough endoplasmic reticulum. The difference in the charges of the 15 residues flanking the first internal signal-anchor determines its orientation, with the more positive portion facing the cytosol. In proteins that span the membrane more than once, the orientation of all subsequent transmembrane segments would be determined by that of the most N-terminal one.
摘要
我们已经制定了一条规则，用于预测在糙面内质网上合成的真核跨膜蛋白中首个内部信号锚定序列的方向。首个内部信号锚定两侧15个残基的电荷差异决定了其方向，电荷更正的部分面向胞质溶胶。在多次跨膜的蛋白质中，所有后续跨膜片段的方向将由最N端的片段决定。
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