el-Gogary S, Leite A, Crivellaro O, Eveleigh D E, el-Dorry H
Department of Biochemistry, University of Sao Paulo, Brazil.
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6138-41. doi: 10.1073/pnas.86.16.6138.
The expression of cellobiohydrolase I mRNA from Trichoderma reesei, measured by Northern blot hybridization, is controlled by the nature of carbon sources used in the culture medium. Cellulose and the soluble disaccharide sophorose, but not glycerol or glucose, act as inducers. Cellobiohydrolase I mRNA was undetectable when antibodies to the major members of the cellulolytic system were present in the culture medium prior to the addition of cellulose. These antibodies had no repressive effect if sophorose was used as an inducer. The results strongly suggest that a low constitutive cellulolytic system catalyzes the formation of a soluble inducer from cellulose and that this inducer triggers the expression of the cellobiohydrolase I gene transcript, most probably at the transcription level.
通过Northern印迹杂交法测定，里氏木霉纤维二糖水解酶I mRNA的表达受培养基中所用碳源性质的控制。纤维素和可溶性二糖槐糖可作为诱导剂，而甘油或葡萄糖则不能。在添加纤维素之前，如果培养基中存在针对纤维素分解系统主要成员的抗体，则无法检测到纤维二糖水解酶I mRNA。如果使用槐糖作为诱导剂，这些抗体没有抑制作用。结果强烈表明，低组成型纤维素分解系统催化从纤维素形成可溶性诱导剂，并且这种诱导剂最有可能在转录水平触发纤维二糖水解酶I基因转录物的表达。
