Abrahão-Neto J, Rossini C H, el-Gogary S, Henrique-Silva F, Crivellaro O, el-Dorry H
Department of Biochemistry, University of São Paulo, Brazil.
Biochemistry. 1995 Aug 22;34(33):10456-62. doi: 10.1021/bi00033a018.
We examined the effects of inhibition of mitochondrial functions on the expression of two nuclear genes encoding the extracellular cellobiohydrolase I (cbh1) and endoglucanase I (egl1) of the cellulase system of the filamentous fungus Trichoderma reesei. The cbh1 and egl1 transcripts are repressed at a low oxygen tension, and by glucose at a concentration known to repress mitochondrial respiration. The transcripts are also down-regulated by chemical agents known to dissipate the proton electrochemical gradient of the inner mitochondrial membrane and blocking of the electron-transport chain, such as DNP and KCN, respectively. These results suggest that expression of those transcripts is influenced by the physiological state of the mitochondria. In addition, heterologous gene fusion shows that the sensitivity of the expression of those transcripts to the functional state of the mitochondria is transcriptionally controlled through the 5'-flanking DNA sequence of those genes.
我们研究了丝状真菌里氏木霉纤维素酶系统中编码胞外纤维二糖水解酶I（cbh1）和内切葡聚糖酶I（egl1）的两个核基因的表达受线粒体功能抑制的影响。cbh1和egl1转录本在低氧张力下以及在已知会抑制线粒体呼吸的葡萄糖浓度下受到抑制。这些转录本也分别被已知会消除线粒体内膜质子电化学梯度和阻断电子传递链的化学试剂（如DNP和KCN）下调。这些结果表明，这些转录本的表达受线粒体生理状态的影响。此外，异源基因融合表明，这些转录本表达对线粒体功能状态的敏感性是通过这些基因的5'侧翼DNA序列进行转录控制的。
