Häggman J, Uimari P
Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland.
J Anim Breed Genet. 2017 Apr;134(2):129-135. doi: 10.1111/jbg.12240. Epub 2016 Sep 13.
Harmful recessive haplotypes for reproductive and fertility traits have previously been detected in cattle, but so far, no studies have been published for pigs. The aim of this study was to locate chromosomal regions with putative lethal haplotypes and estimate the effects of the identified haplotypes on reproductive traits in the Finnish Yorkshire pig breed. We used marker genotypes of 871 Finnish Yorkshire AI boars, genotyped with Illumina's PorcineSNP60 BeadChip. The analysed traits were number of stillborn piglets in first (NSB1) and later (NSB2) parities, total number of piglets born in first and later parities and piglet mortality between birth and weaning in first and later parities. A haplotype was claimed as a putative lethal if it was common in the population, but no homozygous animals were found. We detected altogether 26 putative lethal haplotypes, but only one haplotype on chromosome 8 (position 107.0-113.3 Mb) was significantly associated with traits NSB1 and NSB2. Three possible candidate genes were found in this chromosomal region: MAD2LI, FGF2 and ANXA5. Further analysis is needed to confirm the role of these genes on pig reproductive performance.
此前在牛中已检测到与繁殖和生育性状相关的有害隐性单倍型，但到目前为止，尚未有针对猪的此类研究发表。本研究的目的是定位具有假定致死单倍型的染色体区域，并评估所鉴定单倍型对芬兰约克夏猪繁殖性状的影响。我们使用了871头芬兰约克夏人工授精公猪的标记基因型，这些公猪通过Illumina公司的猪60K SNP芯片进行基因分型。分析的性状包括头胎（NSB1）和经产（NSB2）的死产仔猪数、头胎和经产出生的仔猪总数以及头胎和经产出生至断奶期间的仔猪死亡率。如果一个单倍型在群体中常见但未发现纯合动物，则将其认定为假定致死单倍型。我们总共检测到26个假定致死单倍型，但只有位于8号染色体上（位置107.0 - 113.3 Mb）的一个单倍型与性状NSB1和NSB2显著相关。在该染色体区域发现了三个可能的候选基因：MAD2LI、FGF2和ANXA5。需要进一步分析以确认这些基因对猪繁殖性能的作用。
