Maller Alexandre, de Quadros Thays Cristina Oliveira, Junqueira Otto M, Graña Alfredo Lora, de Lima Montaldi Ana Paula, Alarcon Ricardo Fernandes, Jorge João Atílio, de Lourdes T M Polizeli Maria
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, A. Bandeirantes, 3900, Ribeirão Preto, SP 14040-901 Brazil.
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, SP 16050-680 Brazil.
Springerplus. 2016 Aug 24;5(1):1418. doi: 10.1186/s40064-016-3082-8. eCollection 2016.
Phytases are enzymes that hydrolyze the ester linkage of phytic acid, releasing inositol and inorganic phosphate. The phytic acid (phytate) is a major form of phosphorus in plant foods. Knowing that diet for animal of production has the cereal base (corn and soybean), primarily, broilers need for an alternative to use of the phosphate present in these ingredients, since it does not naturally produce the enzyme phytase, which makes it available. The aims of this work was studding the safe supplementation of Aspergillus japonicus var. Saito crude phytase in feeding broilers and check the biochemical effect on performance and bones of these animals. The enzymatic extract did not have aflatoxins B1, B2, G2 and G1 and zearalenone and ochratoxin, and low concentrations of this extract did not have cytotoxic effects on cells derived from lung tissue. The in vivo experiments showed that the phytase supplied the available phosphate reduction in the broiler feed formulation, with a live weight, weight gain, feed intake, feed conversion, viability, productive efficiency index and carcass yield similar to the control test. Furthermore, the phytase supplementation favored the formation of bone structure and performance of the broilers. The results show the high biotechnological potential of A. japonicus phytase on broiler food supplementation to reduce phosphorus addition in the food formulation. So, this enzyme could be used as a commercial alternative to animal diet supplementation.
植酸酶是一种能水解植酸酯键、释放肌醇和无机磷酸盐的酶。植酸（肌醇六磷酸）是植物性食物中磷的主要存在形式。鉴于生产用动物饲料主要以谷物（玉米和大豆）为基础，肉鸡需要一种替代方法来利用这些饲料成分中的磷，因为它们自身不能自然产生能使磷变得可利用的植酸酶。本研究的目的是研究在肉鸡饲料中安全添加日本曲霉变种斋藤粗植酸酶，并检查其对这些动物生长性能和骨骼的生化影响。该酶提取物不含黄曲霉毒素B1、B2、G2和G1以及玉米赤霉烯酮和赭曲霉毒素，且低浓度的该提取物对源自肺组织的细胞没有细胞毒性作用。体内实验表明，植酸酶可减少肉鸡饲料配方中可利用磷的添加量，其活重、体重增加、采食量、饲料转化率、存活率、生产效率指数和胴体产量与对照试验相似。此外，添加植酸酶有利于肉鸡骨骼结构的形成和生长性能。结果表明，日本曲霉植酸酶在肉鸡饲料补充方面具有很高的生物技术潜力，可减少饲料配方中磷的添加量。因此，这种酶可作为动物饲料补充剂的商业替代品。
