Wu Qiuyun, Yan Weiwen, Han Ruhui, Yang Jingjin, Yuan Jiali, Ji Xiaoming, Liu Yi, Ni Chunhui
Department of Occupational Medicine and Environmental Health, Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
School of Public Health, Xuzhou Medical University, Xuzhou 221004, China.
Int J Environ Res Public Health. 2016 Sep 12;13(9):903. doi: 10.3390/ijerph13090903.
The H19 is a kind of long noncoding RNA, which has been implicated in multiple biological functions. However, the associations between genetic variants in H19 and susceptibility of coal workers' pneumoconiosis (CWP) have been seldom reported. In the present study, three potential polymorphisms (rs2067051, rs217727, and rs2839702) in H19 were genotyped in a case-control study including 703 CWP cases and 705 controls. We found that individuals with the H19 rs2067051 CT/TT genotypes showed a decreased risk of CWP compared with those with the CC genotype (adjusted OR = 0.64, 95%CI = 0.49-0.83, p = 0.001). Further stratified analyses revealed that the associations between variant genotypes of rs2067051 and the risk of CWP were more prominent in subjects of non-smokers (adjusted OR = 0.55, 95%CI = 0.39-0.79, p = 0.001) and CWP patients with Stage I (adjusted OR = 0.63, 95%CI = 0.46-0.86, p = 0.004). Additionally, the protective effects of H19 rs2067051 were also evident in coal miners both with dust exposure years <25 years (adjusted OR = 0.63, 95%CI = 0.42-0.95, p = 0.026) and ≥25 years (adjusted OR = 0.57, 95%CI = 0.40-0.80, p = 0.001). Our results indicated that rs2067051 in the H19 gene is correlated with a deceased risk of CWP in a Chinese population, which may be a potential genetic marker for prevention and intervention of CWP. Further functional studies are warranted to validate our findings.
H19是一种长链非编码RNA，它与多种生物学功能有关。然而，H19基因变异与煤工尘肺（CWP）易感性之间的关联鲜有报道。在本研究中，对703例CWP病例和705例对照进行病例对照研究，对H19基因中的三个潜在多态性位点（rs2067051、rs217727和rs2839702）进行基因分型。我们发现，与CC基因型个体相比，H19 rs2067051 CT/TT基因型个体患CWP的风险降低（校正OR = 0.64，95%CI = 0.49 - 0.83，p = 0.001）。进一步的分层分析显示，rs2067051变异基因型与CWP风险之间的关联在非吸烟受试者中更为显著（校正OR = 0.55，95%CI = 0.39 - 0.79，p = 0.001）以及I期CWP患者中（校正OR = 0.63，95%CI = 0.46 - 0.86，p = 0.004）。此外，H19 rs2067051的保护作用在接尘年限<25年（校正OR = 0.63，95%CI = 0.42 - 0.95，p = 0.026）和≥25年（校正OR = 0.57，95%CI = 0.40 - 0.80，p = 0.001）的煤矿工人中也很明显。我们的结果表明，H19基因中的rs2067051与中国人群中CWP风险降低相关，这可能是CWP预防和干预的潜在遗传标志物。需要进一步的功能研究来验证我们的发现。
