Carraturo Federica, Gargiulo Giuseppe, Giorgio Antonella, Aliberti Francesco, Guida Marco
Laboratory of Hygiene, Dept. of Biology, Univ. of Naples "Federico II,", Via Mezzocannone 16, 80134, Naples, Italy.
J Food Sci. 2016 Oct;81(10):M2545-M2551. doi: 10.1111/1750-3841.13430. Epub 2016 Sep 14.
Recently worldwide food safety authorities indicated the rise of foodborne outbreaks linked to Salmonella: this highlighted the need to intensify monitoring and apply more targeted controls to help manage the spread of the disease. The aim of this study was to assess the prevalence and distribution of Salmonella serotypes in 7 slaughterhouses, located in different areas of Naples province (Regione Campania, Italy). Meat samples collected from the slaughterhouses were submitted for standardized microbiological analysis in 2015. Results of routine testing for Salmonella spp. were analyzed and then compared to biochemical and molecular evaluations. Salmonella spp. were detected in 12% of 320 samples examined (39/320) and the isolation rates ranged from 87% (32 samples) for raw poultry meat to 13% (7 samples) for pork meat. Biochemical serotyping showed that approximately 50% of the isolates belonged to Salmonella enterica serotype Choleraesuis. Rapid detection methods, such as molecular analysis (polymerase chain reaction and gel electrophoresis), able to confirm food matrices contamination, represent a valid support to the fast identification of Salmonella species. A further aspect of the study consisted, indeed, on analyzing isolated strains through molecular evaluations. By amplifying bacterial DNA-using invA primers, selective for Salmonella-it was possible, in less than 3 h, to classify the isolates as Salmonella spp., confirming the results of microbiological outcomes. Results of distribution analysis, supported by rapid molecular approaches, showed the difficulty of reducing Salmonella risk on food chain. This emphasized the importance of periodic surveillance to prevent outbreaks.
最近，全球食品安全当局指出，与沙门氏菌有关的食源性疾病暴发呈上升趋势：这凸显了加强监测和采用更具针对性的控制措施以帮助控制疾病传播的必要性。本研究的目的是评估位于那不勒斯省不同地区（意大利坎帕尼亚大区）的7家屠宰场中沙门氏菌血清型的流行情况和分布。2015年，从这些屠宰场采集的肉类样本被送去进行标准化微生物分析。对沙门氏菌属常规检测的结果进行了分析，然后与生化和分子评估结果进行比较。在所检测的320个样本中，12%（39/320）检测出沙门氏菌属，分离率从生禽肉的87%（32个样本）到猪肉的13%（7个样本）不等。生化血清分型显示，约50%的分离株属于肠炎沙门氏菌猪霍乱血清型。能够确认食品基质污染的快速检测方法，如分子分析（聚合酶链反应和凝胶电泳），是快速鉴定沙门氏菌种类的有效支持。事实上，该研究的另一个方面是通过分子评估分析分离菌株。使用对沙门氏菌具有选择性的invA引物扩增细菌DNA，在不到3小时的时间内就有可能将分离株分类为沙门氏菌属，从而证实微生物检测结果。分布分析结果得到快速分子方法的支持，显示了降低食物链中沙门氏菌风险的难度。这强调了定期监测以预防疾病暴发的重要性。
