Capita R, Alonso-Calleja C, Prieto M
Area de Nutrición y Bromatología, Universidad de León, Ponferrada, Spain.
J Appl Microbiol. 2007 Nov;103(5):1366-75. doi: 10.1111/j.1365-2672.2007.03368.x.
To determine the prevalence of Salmonella enterica serovars in chicken carcasses in slaughterhouses in Spain and to examine genotypic relations among these serovars.
A total of 336 chicken carcasses were collected from six slaughterhouses in Northwestern Spain. Salmonellae were isolated (ISO-6579-1993), serotyped, phage-typed, ribotyped and antibiotyped against 20 antibiotics. Salmonella strains were detected in 60 (17.9%) carcasses. Isolates belonged to nine different serotypes, with Salm. Enteritidis being the most common. Three strains (5%) were resistant to one antibiotic and 24 (40%) were multi-resistant (to more than one antibiotic). The most frequently encountered resistances were to sulphamides, fluoroquinolones and tetracycline. Ribotyping was able to differentiate isolates of the same serotype and phage type.
The Salmonella serotypes and phage types detected are among those most frequently associated with human diseases in Spain. The large percentage of antimicrobial resistant strains is a matter for concern. A high genetic relationship between strains from different slaughterhouses was found.
This study provides detailed information about Salmonella isolates from poultry in Spain. It emphasizes the importance of controlling this pathogen in poultry products, and suggests the need for more prudent use of antibiotics.
确定西班牙屠宰场鸡胴体中肠炎沙门氏菌血清型的流行情况，并研究这些血清型之间的基因型关系。
从西班牙西北部的六个屠宰场收集了总共336份鸡胴体。按照ISO-6579-1993标准分离沙门氏菌，进行血清分型、噬菌体分型、核糖分型，并针对20种抗生素进行抗菌谱分型。在60份（17.9%）胴体中检测到沙门氏菌菌株。分离株属于9种不同的血清型，其中肠炎沙门氏菌最为常见。3株（5%）对一种抗生素耐药，24株（40%）多重耐药（对一种以上抗生素耐药）。最常出现的耐药情况是对磺胺类、氟喹诺酮类和四环素类药物耐药。核糖分型能够区分相同血清型和噬菌体型的分离株。
检测到的沙门氏菌血清型和噬菌体型是西班牙最常与人类疾病相关的类型。抗菌耐药菌株的高比例令人担忧。发现来自不同屠宰场的菌株之间存在高度的遗传关系。
本研究提供了关于西班牙家禽中沙门氏菌分离株的详细信息。强调了在家禽产品中控制这种病原体的重要性，并表明需要更谨慎地使用抗生素。
