A Mouse Model of Fluorescent Protein-expressing Disseminated Peritoneal Lymphoma for Fluorescence-guided Surgery.

作者信息

Matsumoto Takuro, Suetsugu Atsushi, Hasegawa Kosuke, Nakamura Miki, Shibata Yuhei, Aoki Hitomi, Kunisada Takahiro, Tsurumi Hisashi, Shimizu Masahito, Bouvet Michael, Hoffman Robert M

机构信息

Gifu University Graduate School of Medicine, Gifu, Japan.

Gifu University Graduate School of Medicine, Gifu, Japan AntiCancer, Inc., San Diego, CA, U.S.A. Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.

出版信息

Anticancer Res. 2016 Sep;36(9):4483-7. doi: 10.21873/anticanres.10993.

DOI:10.21873/anticanres.10993

PMID:27630285

Abstract

BACKGROUND

Fluorescence-guided surgery (FGS) of tumors is an area of intense development. Peritoneally-disseminated cancer, however, represents a difficult surgical challenge.

MATERIALS AND METHODS

To help meet this challenge, EL4 mouse T-cell lymphoma cells expressing red fluorescent protein (RFP) were injected intraperitoneally in nude mice.

RESULTS

Two weeks after injection of EL4-RFP cells, established peritoneally-disseminated tumors were observed. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). FGS enabled detection of very small peritoneal disseminated tumors and completely resected them in contrast to bright-light which only partially detected the tumors.