Tunca Hatice, Berber Ahmet Ali, Çanakçi Kubra, Tuna Murat, Yildiz Salih Zeki, Aksoy Hüseyin
a Department of Biology , Faculty of Arts and Sciences, Sakarya University , Esentepe Campus , Sakarya , Turkey and.
b Department of Chemistry , Faculty of Arts and Sciences, Sakarya University , Esentepe Campus , Sakarya , Turkey.
Drug Chem Toxicol. 2017 Jul;40(3):300-308. doi: 10.1080/01480545.2016.1223094. Epub 2016 Sep 15.
In this study, a Cd(II) complex was synthesized using 8-hydroxyquinoline and thiocyanate as the ligands and structurally characterized with the combination of FTIR, H-NMR, C-NMR, UV-vis, and MS spectral data. Then, genotoxic effects of the prepared complex were investigated. Genotoxic properties of the dimeric 8-hydroxyquinolinthiocyanatoCd(II) [Cd(8Q)(SCN)] complex synthesized as drug raw material were analyzed in human peripheral blood lymphocytes. Concentrations of 1, 2, 4, 6, and 8 μg/mL [Cd(8Q)(SCN)] were used for 24 and 48 h durations. [Cd(8Q)(SCN)] significantly increased chromosomal aberrations (CAs) at 4, 6, and 8 μg/mL concentrations after a 24- h period and 2 and 4 μg/mL after a 48-h period. [Cd(8Q)(SCN)] significantly decreased the mitotic index (MI) at all concentrations, both at 24 and 48 h. Micronuclei frequency (MN) was not affected by [Cd(8Q)(SCN)] treatment compared with the control. After application for a 48 h period, 6 and 8 μg/mL concentrations showed toxic effects both in chromosomal abnormality and in micronucleus tests. It also decreased the cytokinesis-block proliferation index (CBPI), but this result was statistically significant only at 6 and 8 μg/mL concentrations. In the comet assay (single-cell gel electrophoresis (SCGE)), significant increases in comet tail length, tail moment, and tail intensity were observed at all concentrations. [Cd(8Q)(SCN)] displays clastogenic effect in the concentrations used in human peripheral lymphocytes at chromosomal abnormality, micronucleus tests, and cytokinesis-block proliferation index parameters. Further studies should be conducted in other test systems to evaluate the complete genotoxic potential of [Cd(8Q)(SCN)].
在本研究中，以8-羟基喹啉和硫氰酸盐作为配体合成了一种镉（II）配合物，并通过傅里叶变换红外光谱（FTIR）、氢核磁共振（H-NMR）、碳核磁共振（C-NMR）、紫外可见光谱（UV-vis）和质谱（MS）光谱数据相结合的方式对其结构进行了表征。然后，研究了所制备配合物的遗传毒性效应。分析了作为药物原料合成的二聚体8-羟基喹啉硫氰酸镉（II）[Cd(8Q)(SCN)]配合物在人外周血淋巴细胞中的遗传毒性特性。使用浓度为1、2、4、6和8μg/mL的[Cd(8Q)(SCN)]，作用时间为24小时和48小时。在24小时后，[Cd(8Q)(SCN)]在浓度为4、6和8μg/mL时显著增加了染色体畸变（CA），在48小时后，浓度为2和4μg/mL时显著增加了染色体畸变。[Cd(8Q)(SCN)]在所有浓度下，在24小时和48小时时均显著降低了有丝分裂指数（MI）。与对照组相比，[Cd(8Q)(SCN)]处理对微核频率（MN）没有影响。在处理48小时后，6和8μg/mL的浓度在染色体异常和微核试验中均显示出毒性作用。它还降低了胞质分裂阻滞增殖指数（CBPI），但这一结果仅在6和8μg/mL浓度时具有统计学意义。在彗星试验（单细胞凝胶电泳（SCGE））中，在所有浓度下均观察到彗星尾长、尾矩和尾强度显著增加。[Cd(8Q)(SCN)]在用于人外周淋巴细胞的浓度下，在染色体异常、微核试验和胞质分裂阻滞增殖指数参数方面表现出致断裂效应。应在其他测试系统中进行进一步研究，以评估[Cd(8Q)(SCN)]的完整遗传毒性潜力。
