Malhotra Jyoti, Waterboer Tim, Pawlita Michael, Michel Angelika, Cai Qiuyin, Zheng Wei, Gao Yu-Tang, Lan Qing, Rothman Nathaniel, Langseth Hilde, Grimsrud Tom K, Yuan Jian-Min, Koh Woon-Puay, Wang Renwei, Arslan Alan A, Zeleniuch-Jacquotte Anne, Boffetta Paolo
Icahn School of Medicine at Mount Sinai, 17 East 102 St, Floor 4 West, Room 110, New York, NY, USA.
Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA.
Br J Cancer. 2016 Oct 25;115(9):1131-1139. doi: 10.1038/bjc.2016.285. Epub 2016 Sep 15.
Lung cancer in never smokers is a significant contributor of cancer mortality worldwide. In this analysis, we explored the role of nine human polyomaviruses, including JC virus (JCV), BK virus (BKV) and Merkel cell virus (MCV), in lung cancer development in never smokers as there are data to support that polyomaviruses are potentially carcinogenic in the human lung.
We used multiplex serology to detect serum antibodies to polyomaviruses in a nested case-control design combining lung cancer cases and controls from four cohort studies - NYU Women's Health Study (NYU-WHS), Janus Serum Bank, Shanghai Women's Health Study and Singapore Chinese Health Study (SCHS).
The final analyses included 511 cases and 508 controls. Seroprevalence for each polyomavirus showed significant heterogeneity by study, but overall there were no statistically significant differences between cases and controls. In total, 69.1% of the cases and 68.7% of the controls were seropositive for JCV VP1 antibody. Seropositivity for BKV was higher at 89.0% in cases and 89.8% in controls and lower for MCV at 59.3% in cases and 61.6% in controls. Similar results were obtained after adding an additional retrospective case-control study (Xuanwei study) to the analysis.
Our results do not support the hypothesis that seropositivity for polyomaviruses is associated with increased lung cancer risk in never smokers. Future research to evaluate relationship between polyomavirus infection and lung carcinogenesis should focus more on evaluating the presence of virus or viral nucleic acids (DNA or RNA) in lung tumour samples.
非吸烟人群中的肺癌是全球癌症死亡的重要原因。在本分析中，我们探讨了九种人类多瘤病毒，包括JC病毒（JCV）、BK病毒（BKV）和默克尔细胞病毒（MCV）在非吸烟人群肺癌发生中的作用，因为有数据支持多瘤病毒在人类肺部具有潜在致癌性。
我们采用多重血清学方法，在一项巢式病例对照研究中检测血清中针对多瘤病毒的抗体，该研究结合了来自四项队列研究（纽约大学女性健康研究（NYU-WHS）、雅努斯血清库、上海女性健康研究和新加坡华人健康研究（SCHS））的肺癌病例和对照。
最终分析纳入了511例病例和508例对照。各多瘤病毒的血清阳性率在不同研究中显示出显著异质性，但总体而言，病例组和对照组之间无统计学显著差异。总体上，69.1%的病例和68.7%的对照JCV VP1抗体呈血清阳性。BKV的血清阳性率在病例组中较高，为89.0%，在对照组中为89.8%；MCV的血清阳性率较低，病例组为59.3%，对照组为61.6%。在分析中加入另一项回顾性病例对照研究（宣威研究）后，得到了类似结果。
我们的结果不支持多瘤病毒血清阳性与非吸烟人群肺癌风险增加相关的假设。未来评估多瘤病毒感染与肺癌发生关系的研究应更多地集中于评估肺肿瘤样本中病毒或病毒核酸（DNA或RNA）的存在情况。
