Tan Weng Chun, Mat Isa Nor Ashidi
Imaging and Intelligent Systems Research Team (ISRT), School of Electrical and Electronic Engineering, Universiti Sains Malaysia, Nibong Tebal, Penang, Malaysia.
PLoS One. 2016 Sep 15;11(9):e0162985. doi: 10.1371/journal.pone.0162985. eCollection 2016.
In human sperm motility analysis, sperm segmentation plays an important role to determine the location of multiple sperms. To ensure an improved segmentation result, the Laplacian of Gaussian filter is implemented as a kernel in a pre-processing step before applying the image segmentation process to automatically segment and detect human spermatozoa. This study proposes an intersecting cortical model (ICM), which was derived from several visual cortex models, to segment the sperm head region. However, the proposed method suffered from parameter selection; thus, the ICM network is optimised using particle swarm optimization where feature mutual information is introduced as the new fitness function. The final results showed that the proposed method is more accurate and robust than four state-of-the-art segmentation methods. The proposed method resulted in rates of 98.14%, 98.82%, 86.46% and 99.81% in accuracy, sensitivity, specificity and precision, respectively, after testing with 1200 sperms. The proposed algorithm is expected to be implemented in analysing sperm motility because of the robustness and capability of this algorithm.
在人类精子活力分析中,精子分割对于确定多个精子的位置起着重要作用。为了确保获得更好的分割结果,在应用图像分割过程自动分割和检测人类精子之前,高斯拉普拉斯滤波器在预处理步骤中被用作内核。本研究提出了一种相交皮层模型(ICM),该模型源自多个视觉皮层模型,用于分割精子头部区域。然而,所提出的方法存在参数选择问题;因此,使用粒子群优化对ICM网络进行优化,其中引入特征互信息作为新的适应度函数。最终结果表明,所提出的方法比四种最先进的分割方法更准确、更稳健。在用1200个精子进行测试后,所提出的方法在准确率、灵敏度、特异性和精确率方面分别达到了98.14%、98.82%、86.46%和99.81%。由于该算法的稳健性和能力,预计所提出的算法将用于分析精子活力。