用具有不同官能团的酶合成序列定义的合成核酸聚合物。
Enzymatic Synthesis of Sequence-Defined Synthetic Nucleic Acid Polymers with Diverse Functional Groups.
机构信息
Department of Chemistry, University of Georgia, Athens, GA, 30602, USA.
出版信息
Angew Chem Int Ed Engl. 2016 Oct 10;55(42):13164-13168. doi: 10.1002/anie.201607538.
Abstract
The development and in-depth analysis of T4 DNA ligase-catalyzed DNA templated oligonucleotide polymerization toward the generation of diversely functionalized nucleic acid polymers is described. The NNNNT codon set enables low codon bias, high fidelity, and high efficiency for the polymerization of ANNNN libraries comprising various functional groups. The robustness of the method was highlighted in the copolymerization of a 256-membered ANNNN library comprising 16 sub-libraries modified with different functional groups. This enabled the generation of diversely functionalized synthetic nucleic acid polymer libraries with 93.8 % fidelity. This process should find ready application in DNA nanotechnology, DNA computing, and in vitro evolution of functional nucleic acid polymers.
摘要
描述了 T4 DNA 连接酶催化的 DNA 模板寡核苷酸聚合反应的发展和深入分析，以生成具有多种功能化的核酸聚合物。NNNNT 密码子集可实现低密码子偏向性、高保真度和高效率，用于聚合包含各种官能团的 ANNNN 文库。该方法的稳健性在包含 16 个子文库的 256 成员的 ANNNN 文库的共聚中得到了突出体现，这些子文库用不同的官能团进行了修饰。这使得具有 93.8%保真度的多样化功能化合成核酸聚合物文库得以生成。该过程应在 DNA 纳米技术、DNA 计算和功能核酸聚合物的体外进化中得到广泛应用。
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本文引用的文献
1
Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors.生物系统中的多价相互作用：对多价配体和抑制剂设计与应用的启示
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2
Generation of Synthetic Copolymer Libraries by Combinatorial Assembly on Nucleic Acid Templates.通过在核酸模板上进行组合组装生成合成共聚物文库。
ACS Comb Sci. 2016 Jul 11;18(7):355-70. doi: 10.1021/acscombsci.6b00059. Epub 2016 Jun 17.
3
Enhancing aptamer function and stability via in vitro selection using modified nucleic acids.通过使用修饰核酸的体外筛选提高适体功能和稳定性。
Methods. 2016 Aug 15;106:29-36. doi: 10.1016/j.ymeth.2016.03.008. Epub 2016 Mar 21.
4
DNA-Catalyzed Amide Hydrolysis.DNA催化的酰胺水解
J Am Chem Soc. 2016 Feb 24;138(7):2106-9. doi: 10.1021/jacs.5b12647. Epub 2016 Feb 15.
5
A High-Fidelity Codon Set for the T4 DNA Ligase-Catalyzed Polymerization of Modified Oligonucleotides.用于T4 DNA连接酶催化修饰寡核苷酸聚合的高保真密码子集。
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6
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J Am Chem Soc. 2015 Sep 2;137(34):11191-6. doi: 10.1021/jacs.5b07675. Epub 2015 Aug 21.
7
Nucleic Acid Ligands With Protein-like Side Chains: Modified Aptamers and Their Use as Diagnostic and Therapeutic Agents.具有类蛋白侧链的核酸配体：修饰的适体及其作为诊断和治疗剂的用途。
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8
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Chem Rev. 2014 Mar 12;114(5):2881-941. doi: 10.1021/cr400354z. Epub 2014 Feb 27.
9
Sequence-controlled polymers.序列控制聚合物。
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10
Toward the combinatorial selection of chemically modified DNAzyme RNase A mimics active against all-RNA substrates.针对所有 RNA 底物具有催化活性的化学修饰 DNAzyme RNase A 模拟物的组合选择。
ACS Comb Sci. 2013 Apr 8;15(4):174-82. doi: 10.1021/co3001378. Epub 2013 Mar 13.
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