Department of Chemistry, University of Georgia, Athens, GA, 30602, USA.
Angew Chem Int Ed Engl. 2016 Oct 10;55(42):13164-13168. doi: 10.1002/anie.201607538.
The development and in-depth analysis of T4 DNA ligase-catalyzed DNA templated oligonucleotide polymerization toward the generation of diversely functionalized nucleic acid polymers is described. The NNNNT codon set enables low codon bias, high fidelity, and high efficiency for the polymerization of ANNNN libraries comprising various functional groups. The robustness of the method was highlighted in the copolymerization of a 256-membered ANNNN library comprising 16 sub-libraries modified with different functional groups. This enabled the generation of diversely functionalized synthetic nucleic acid polymer libraries with 93.8 % fidelity. This process should find ready application in DNA nanotechnology, DNA computing, and in vitro evolution of functional nucleic acid polymers.
描述了 T4 DNA 连接酶催化的 DNA 模板寡核苷酸聚合反应的发展和深入分析，以生成具有多种功能化的核酸聚合物。NNNNT 密码子集可实现低密码子偏向性、高保真度和高效率，用于聚合包含各种官能团的 ANNNN 文库。该方法的稳健性在包含 16 个子文库的 256 成员的 ANNNN 文库的共聚中得到了突出体现，这些子文库用不同的官能团进行了修饰。这使得具有 93.8%保真度的多样化功能化合成核酸聚合物文库得以生成。该过程应在 DNA 纳米技术、DNA 计算和功能核酸聚合物的体外进化中得到广泛应用。
