Zhu Zhong-Jiao, Huang Peng, Chong Yan-Xue, Kang Li-Xin, Huang Xing, Zhu Zhong-Xiu, Nie Lin
Department of Orthopedics, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China.
Department of Emergency, Tengzhou Hospital of Traditional Chinese Medicine, Tengzhou, Shandong 277500, P.R. China.
Mol Med Rep. 2016 Nov;14(5):4271-4278. doi: 10.3892/mmr.2016.5741. Epub 2016 Sep 14.
MicroRNA-181a (miR-181a) is upregulated in osteosarcoma, and its overexpression promotes the proliferation and inhibits the apoptosis of osteosarcoma cells. However, the mechanism of miR‑181a as an oncogene remains to be fully elucidated in osteosarcoma. Cleavage factor (CF) Im25 links alternative polyadenylation to glioblastoma tumor suppression, however, its role in osteosarcoma has not been reported. In the present study, it was confirmed that the expression of miR‑181a was upregulated in osteosarcoma, and that silencing miR‑181a inhibited the proliferation and promoted the apoptosis of osteosarcoma cells. miRNAs are short non‑coding RNAs, which regulate target mRNAs by binding predominantly to the 3'untranslated region (3'UTR), inducing either translational repression or degradation of the target. In the present study, target genes of miR‑181a were screened using miRanda, which is a commonly used prediction algorithm. It was found that miR‑181a targeted the 3'UTR of CFIm25 mRNA. Subsequent experiments confirmed that miR‑181a downregulated the expression of CFIm25 in osteosarcoma cells. Finally, it was demonstrated that the CFIm25 protein was also downregulated in osteosarcoma tissues, and inhibited the proliferation and promoted the apoptosis of the cells. Elucidating the roles of miR‑181a and CFIm25 in osteosarcoma not only assists in further understanding the pathogenesis and progression of this disease, but also offers novel targets for effective therapies.
微小RNA-181a（miR-181a）在骨肉瘤中表达上调，其过表达促进骨肉瘤细胞增殖并抑制其凋亡。然而，miR-181a作为癌基因在骨肉瘤中的作用机制仍有待充分阐明。切割因子（CF）Im25将可变聚腺苷酸化与胶质母细胞瘤肿瘤抑制联系起来，然而，其在骨肉瘤中的作用尚未见报道。在本研究中，证实了miR-181a在骨肉瘤中表达上调，沉默miR-181a可抑制骨肉瘤细胞增殖并促进其凋亡。微小RNA是短的非编码RNA，主要通过与3'非翻译区（3'UTR）结合来调节靶mRNA，从而诱导靶标的翻译抑制或降解。在本研究中，使用常用的预测算法miRanda筛选miR-181a的靶基因。发现miR-181a靶向CFIm25 mRNA的3'UTR。随后的实验证实，miR-181a下调骨肉瘤细胞中CFIm25的表达。最后，证明CFIm25蛋白在骨肉瘤组织中也下调，并抑制细胞增殖并促进其凋亡。阐明miR-181a和CFIm25在骨肉瘤中的作用不仅有助于进一步了解该疾病的发病机制和进展，还为有效治疗提供了新的靶点。
