虎鲨的微卫星位点及焦磷酸测序技术的跨物种扩增
Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology.
作者信息
Mendes Natália J, Cruz Vanessa P, Ashikaga Fernando Y, Camargo Sâmia M, Oliveira Claudio, Piercy Andrew N, Burgess George H, Coelho Rui, Santos Miguel N, Mendonça Fernando F, Foresti Fausto
机构信息
Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil.
Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil; Laboratório de Genética Pesqueira e Conservação, Institute of Marine Sciences, Universidade Federal de São Paulo, Santos, São Paulo, Brazil.
出版信息
PeerJ. 2016 Aug 30;4:e2205. doi: 10.7717/peerj.2205. eCollection 2016.
The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.
虎鲨（居氏鼬鲨）分布于全球热带和暖温带海域，在世界各地的众多渔业活动中被捕获，主要作为兼捕渔获物。目前，国际自然保护联盟（IUCN）红色名录将其评估为近危物种。在本研究中，我们利用来自西大西洋的29个样本，通过下一代测序（454焦磷酸测序）鉴定出9个微卫星位点。对这些位点的遗传多样性进行评估，结果显示共有48个等位基因，每个位点的等位基因数在3至7个之间（平均为5.3个）。大多数位点对其他物种如长鳍真鲨、尖吻鲭鲨和大眼长尾鲨成功进行了跨物种扩增。鉴于遗传标记在生物保护方面的潜在适用性，这些数据可能有助于对全球范围内该物种及其他鲨鱼物种进行种群评估。
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