[用二甲基亚甲基蓝间接分光光度法微量测定硫酸化糖胺聚糖]
[Indirect spectrophotometric microdetermination of sulfated glycosaminoglycans with dimethylmethylene blue].
作者信息
Müller G, Hanschke M
出版信息
Z Med Lab Diagn. 1989;30(3):180-5.
PMID:2763629
Abstract
A microanalysis technique has been developed for the quantitative estimation of proteoglycans in cartilage extracts measuring the spectrophotometric decrease of absorbance of the dye dimethylmethylene blue after complexing with sulfated glycosaminoglycans at 590 nm. The reproducibility of the method is shown investigating the influence of different substances.
摘要
已开发出一种微量分析技术，用于定量评估软骨提取物中的蛋白聚糖，该技术通过测量染料二甲基亚甲基蓝在590nm处与硫酸化糖胺聚糖络合后吸光度的分光光度下降来实现。通过研究不同物质的影响，展示了该方法的重现性。
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