Farndale R W, Buttle D J, Barrett A J
Biochim Biophys Acta. 1986 Sep 4;883(2):173-7. doi: 10.1016/0304-4165(86)90306-5.
The dimethylmethylene blue assay for sulphated glycosaminoglycans has found wide acceptance as a quick and simple method of measuring the sulphated glycosaminoglycan content of tissues and fluids. The available assay methods have lacked specificity for sulphated glycosaminoglycans in the presence of other polyanions, however, and have not discriminated between the different sulphated glycosaminoglycans. We now describe a modified form of the dimethylmethylene blue assay that has improved specificity for sulphated glycosaminoglycans, and we show that in conjunction with specific polysaccharidases, the dimethylmethylene blue assay can be used to quantitate individual sulphated glycosaminoglycans.
用于检测硫酸化糖胺聚糖的二甲基亚甲基蓝分析法，作为一种快速简便的测量组织和体液中硫酸化糖胺聚糖含量的方法，已被广泛接受。然而，现有的检测方法在存在其他聚阴离子的情况下，对硫酸化糖胺聚糖缺乏特异性，并且无法区分不同的硫酸化糖胺聚糖。我们现在描述一种改良的二甲基亚甲基蓝分析法，它对硫酸化糖胺聚糖具有更高的特异性，并且我们证明，结合特定的多糖酶，二甲基亚甲基蓝分析法可用于定量分析单个硫酸化糖胺聚糖。
