Sabiston P, Adams M E, Ho Y A
Anal Biochem. 1985 Sep;149(2):543-8. doi: 10.1016/0003-2697(85)90611-6.
The 1,9-dimethylmethylene blue dye-binding assay for sulfated glycosaminoglycans has been modified and adapted for use on a Technicon autoanalyzer. The assay was linear for chondroitin sulfate from 2.5 to 500 micrograms/ml and used only 2.5 microliters sample. Interference by salts and solvents commonly used in proteoglycan research was minimal and could be compensated for by using standards in the appropriate solvent. High concentrations of albumin and serum interfered strongly but most of the inhibition could be overcome by papain digestion.
用于硫酸化糖胺聚糖的1,9 - 二甲基亚甲基蓝染料结合测定法已被改进并适用于Technicon自动分析仪。该测定法对于硫酸软骨素在2.5至500微克/毫升范围内呈线性，且仅使用2.5微升样品。蛋白聚糖研究中常用的盐和溶剂的干扰极小，可通过在适当溶剂中使用标准品来补偿。高浓度的白蛋白和血清干扰强烈，但大部分抑制作用可通过木瓜蛋白酶消化来克服。
