Wei Jing-Jing, Tang Du-Peng, Xie Jing-Jing, Yang Li-Yong, Zhuang Ze-Hao
Department of Gastroenterology, the First Affiliated Hospital of Fujian Medical University, China.
Department of Endocrinology, the First Affiliated Hospital of Fujian Medical University, China.
Prostaglandins Leukot Essent Fatty Acids. 2016 Sep;112:37-43. doi: 10.1016/j.plefa.2016.08.003. Epub 2016 Aug 16.
To investigate the effect of dietary ratio of n-6/n-3 PUFAs on chronic reflux esophagitis (RE) and lipid peroxidation.
Rat RE model were established and then fed on a diet contained different n-6/n-3 PUFA ratios (1:1.5, 5:1, 10:1) or received pure n-6 PUFA diet for 14 days. Esophageal pathological changes were evaluated using macroscopic examination and hematoxyline-eosin staining. IL-1β, IL-8, and TNFα mRNA and protein levels of were determined using RT-PCR and Western blotting, respectively. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined using ELISA.
The severity of esophagitis was lowest in the PUFA(1:1.5) group (P<0.05). IL-1β, IL-8, and TNFα mRNA and protein and MDA levels were significantly increased in model groups with the increasing n-6/n-3 PUFA ratios. SOD levels were significantly decreased in all RE PUFA groups (P<0.05).
Esophageal injury and lipid peroxidation appeared to be ameliorated by increased n-3 PUFAs intake.
研究膳食中n-6/n-3多不饱和脂肪酸（PUFAs）比例对慢性反流性食管炎（RE）及脂质过氧化的影响。
建立大鼠RE模型，然后分别给予不同n-6/n-3 PUFA比例（1:1.5、5:1、10:1）的饮食或纯n-6 PUFA饮食14天。通过大体检查和苏木精-伊红染色评估食管病理变化。分别采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法测定白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）和肿瘤坏死因子α（TNFα）的mRNA和蛋白质水平。采用酶联免疫吸附测定法（ELISA）测定丙二醛（MDA）和超氧化物歧化酶（SOD）水平。
PUFA（1:1.5）组食管炎严重程度最低（P<0.05）。随着n-6/n-3 PUFA比例增加，模型组中IL-1β、IL-8和TNFα的mRNA、蛋白质以及MDA水平显著升高。所有RE PUFA组的SOD水平均显著降低（P<0.05）。
增加n-3 PUFAs摄入量似乎可改善食管损伤和脂质过氧化。
