Zhuang Ze-Hao, Xie Jing-Jing, Wei Jing-Jing, Tang Du-Peng, Yang Li-Yong
Department of Endoscopy, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou, 35005, China.
Department of Endocrinology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 35005, China.
Lipids Health Dis. 2016 Oct 4;15(1):172. doi: 10.1186/s12944-016-0332-2.
Polyunsaturated fatty acids (PUFAs) play various roles in inflammation. However, the effect of PUFAs in the development of reflux esophagitis (RE) is unclear. This study is to investigate the potential effect of n-3/n-6 PUFAs on acute RE in rats along with the underlying protective mechanisms.
Forty Sprague Dawley rats were randomly divided into four groups (n = 10 in each group). RE model was established by pyloric clip and section ligation. Fish oil- and soybean oil-based fatty emulsion (n-3 and n-6 groups), or normal saline (control and sham operation groups) was injected intraperitoneally 2 h prior to surgery and 24 h postoperatively (2 mL/kg, respectively). The expressions of interleukin (IL)-1β, IL-8, IL-6 and myeloid differentiation primary response gene 88 (MyD88) in esophageal tissues were evaluated by Western blot and immunohistochemistry after 72 h. The malondialdehyde (MDA) and superoxide dismutase (SOD) expression in the esophageal tissues were determined to assess the oxidative stress.
The mildest macroscopic/microscopic esophagitis was found in the n-3 group (P < 0.05). The expression of IL-1β, IL-8, IL-6 and MyD88 were increased in all RE groups, while the lowest and highest expression were found in n-3 and n-6 group, respectively (P < 0.05). The MDA levels were increased in all groups (P < 0.05), in an ascending trend from n-3, n-6 groups to control group. The lowest and highest SOD levels were found in the control and n-3 group, respectively (P < 0.05).
n-3 PUFAs may reduce acute RE in rats, which may be due to inhibition of the MyD88-NF-kB pathway and limit oxidative damage.
多不饱和脂肪酸（PUFAs）在炎症中发挥多种作用。然而，PUFAs在反流性食管炎（RE）发生发展中的作用尚不清楚。本研究旨在探讨n-3/n-6 PUFAs对大鼠急性RE的潜在影响及其潜在的保护机制。
将40只Sprague Dawley大鼠随机分为四组（每组n = 10）。通过幽门夹闭和结扎建立RE模型。在手术前2小时和术后24小时腹腔注射鱼油和大豆油基脂肪乳剂（n-3和n-6组），或生理盐水（对照组和假手术组）（分别为2 mL/kg）。72小时后，通过蛋白质免疫印迹法和免疫组织化学法评估食管组织中白细胞介素（IL）-1β、IL-8、IL-6和髓样分化初级反应基因88（MyD88）的表达。测定食管组织中丙二醛（MDA）和超氧化物歧化酶（SOD）的表达以评估氧化应激。
n-3组的宏观/微观食管炎最轻（P < 0.05）。所有RE组中IL-1β、IL-8、IL-6和MyD88的表达均增加，而n-3组和n-6组中分别发现最低和最高表达（P < 0.05）。所有组的MDA水平均升高（P < 0.05），从n-3组、n-6组到对照组呈上升趋势。对照组和n-3组中分别发现最低和最高的SOD水平（P < 0.05）。
n-3 PUFAs可能减轻大鼠急性RE，这可能是由于抑制了MyD88-NF-kB途径并限制了氧化损伤。
