Pabisch Silvia, Akabane Chika, Wagermaier Wolfgang, Roschger Andreas, Ogura Taku, Hyodo Ryo, Kataoka Shinsuke, Tobori Norio, Okano Tomomichi, Murakami Shinya, Fratzl Peter, Weinkamer Richard
Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany; Functional Materials Science Research Laboratories, Research & Development Headquarters, LION Corporation, Tokyo, Japan.
J Struct Biol. 2016 Nov;196(2):223-231. doi: 10.1016/j.jsb.2016.09.007. Epub 2016 Sep 13.
Alveolar bone - the bony ridge containing the tooth sockets - stands out by its remodeling activity where bone is being formed and resorbed at a much higher rate than in any other bony tissue. Teeth that are anchored in the jaw through the periodontal ligament exert very large localized loads during mastication that could lead to a unique adaptation of the collagen/mineral structure in the bone. Our aim was to characterize the nanostructure of alveolar bone and to determine the influence of diabetes on structural characteristics of the mineralized matrix. Using small- and wide-angle X-ray scattering (SAXS/WAXS), we studied a spontaneous diabetic mouse model (KK+) and its corresponding healthy controls (KK-) (n=6) to determine the size and mutual alignment of the mineral nanoparticles embedded in the collagen matrix. On cross-sections (buccal-lingual) of the first molar multiple line scans with a spatial resolution of 30μm were performed on each sample, from the lingual to the buccal side of the mandible. Mineral particle thickness and length are decreasing towards the tooth in both buccal and lingual sides of alveolar bone. While mineral particles are well aligned with the long axis of the tooth on the buccal side, they are in a quarter of the measurements oriented along two preferred directions on the lingual side. These nanostructural differences can be interpreted as the result of an asymmetric loading during mastication, leading to a tilting of the tooth in its socket. In diabetic mice particle thicknesses are smaller compared to control animals.
牙槽骨——包含牙槽窝的骨嵴——因其重塑活动而突出，在牙槽骨中，骨的形成和吸收速度比任何其他骨组织都要快得多。通过牙周韧带固定在颌骨中的牙齿在咀嚼过程中会施加非常大的局部负荷，这可能导致骨中胶原蛋白/矿物质结构的独特适应性变化。我们的目的是表征牙槽骨的纳米结构，并确定糖尿病对矿化基质结构特征的影响。我们使用小角和广角X射线散射（SAXS/WAXS），研究了自发糖尿病小鼠模型（KK+）及其相应的健康对照（KK-）（n = 6），以确定嵌入胶原蛋白基质中的矿物质纳米颗粒的大小和相互排列。在每个样本的第一磨牙颊舌向横截面上，从下颌骨的舌侧到颊侧进行了空间分辨率为30μm的多条线扫描。在牙槽骨的颊侧和舌侧，矿物质颗粒的厚度和长度都朝着牙齿方向减小。虽然颊侧的矿物质颗粒与牙齿的长轴排列良好，但在舌侧，四分之一的测量结果显示它们沿两个优选方向排列。这些纳米结构差异可以解释为咀嚼过程中不对称负荷的结果，导致牙齿在牙槽窝中倾斜。与对照动物相比，糖尿病小鼠的颗粒厚度较小。
