Magnoli A P, Rodriguez M C, Poloni V L, Rojo M C, Combina M, Chiacchiera S M, Dalcero A M, Cavaglieri L R
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires (CABA), Argentina.
J Appl Microbiol. 2016 Dec;121(6):1766-1776. doi: 10.1111/jam.13297.
To isolate and characterize native yeast strains from broilers' environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B (AFB ).
A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB binding percentages varied among yeast strains and with AFB concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 10 cells per ml and 100 ng ml of AFB respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB -yeast complex demonstrated the irreversibility of the binding process.
Yeast strains tested in this study constitute potential AFB adsorbents and they possess the advantage to be native from the avian environment.
This study makes a contribution to using native yeasts from broilers' environment for controlling chronic aflatoxicosis in avian production.
从肉鸡的饲料、粪便和肠道环境中分离并鉴定天然酵母菌株，并评估它们对黄曲霉毒素B（AFB）的结合能力。
共分离出9株酵母菌株：3株来自饲料，鉴定为季也蒙毕赤酵母（2株）和葡萄牙棒孢酵母（1株）；2株来自肠道，鉴定为热带假丝酵母；4株来自粪便，鉴定为葡萄牙棒孢酵母（3株）和法比安丝孢酵母（1株）。AFB结合率因酵母菌株和AFB浓度而异。为进行吸附研究，从每个属和每个来源中各选择1株菌株如下：来自饲料的葡萄牙棒孢酵母和季也蒙毕赤酵母，来自粪便的葡萄牙棒孢酵母和法比安丝孢酵母，以及来自肠道的热带假丝酵母。细胞和毒素的最适浓度分别为每毫升10个细胞和每毫升100纳克AFB。所有测试的酵母菌株均表现出相似的吸附能力，与来源无关。所有测定酵母的吸附等温线研究均显示为L型或朗缪尔行为，且对毒素的亲和力各不相同。AFB-酵母复合物的稳定性证明了结合过程的不可逆性。
本研究中测试的酵母菌株构成了潜在的AFB吸附剂，并且具有来自禽类环境的天然优势。
本研究为利用肉鸡环境中的天然酵母控制禽类生产中的慢性黄曲霉毒素中毒做出了贡献。
